Platycladus orientalis shells promote melanogenesis via p38,AKT and Wnt/β-catenin signaling pathways  

作　　者：Fu-Rui Liu Hao Xiang Qian-Bei Guo Shu-Li Man Long Ma 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Key Laboratory of Industrial Microbiology,Ministry of Education,Tianjin Key Laboratory of Industry Microbiology,National and Local United Engineering Lab of Metabolic Control Fermentation Technology,China International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal Chemistry,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China

出　　处：《Traditional Medicine Research》2022年第6期56-64,共9页TMR传统医学研究

基　　金：National Natural Science Foundation of China(No.82074069,No.32072309).

摘　　要：Background:Platycladus orientalis,which has been employed in traditional Chinese medicine for cool blood,antibacterial,promotion of hair growth and therapy of poliosis for centuries.However,there have been few reports focusing on Platycladus orientalis shells treating pigmentary disorders.Methods:In present study,gas chromatography–mass spectrometry was applied to analyzing the volatile composition of Platycladus orientalis shells and cellular metabolism.Experiments in vivo were carried out to evaluate the effect of Platycladus orientalis shells treating pigmentation disorders in C57BL/6J mice.Results:Our results indicated that cedrol occupied the largest percentage in Platycladus orientalis shells(17.1%).Meanwhile,Platycladus orientalis shells up-regulated the content of palmitic acid and increased melanin content and tyrosinase activity.Its mechanism possibly involved in the inhibiting phosphorylation of AKT and β-catenin,increasing phosphorylation of p38 to promote microphthalmia-associated transcription factor expression.The animal experiment also proved that Platycladus orientalis shells promoted melanogensis and hair blacken in C57BL/6J mice.Conclusion:All in all,Platycladus orientalis shells potentially promoted melanogenesis in vitro and in vivo.Cedrol was regarded as the main active substance in Platycladus orientalis shells.Therefore,it could be used for treatment of pigmentary disorders under safe concentration in the prospective application.

关 键 词：GC-MS Platycladus orientalis shells MELANOGENESIS TYROSINASE pigmentary disorders 

分 类 号：R284[医药卫生—中药学]