宁乡猪FABPs、SLC13A5和NR1H4基因多态性及其与IMF含量的关联分析  

Polymorphisms of FABPs,SLC13A5 and NR1H4 Genes in Ningxiang Pigs and Their Association with IMF Content

作　　者：李艺阳 殷诗舒 廖印长 徐康[2,3] 张跃博 何俊 LI Yiyang;YIN Shishu;LIAO Yinchang;XU Kang;ZHANG Yuebo;HE Jun(College of Animal Science and Technology,Hunan Agricultural University,Changsha 410000,China;Laboratory of Animal Nutrition Physiology and Metabolic Processes,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410000,China;Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510000,China)

机构地区：[1]湖南农业大学动物科学技术学院,长沙410000 [2]中国科学院亚热带农业生态研究所动物营养生理与代谢过程实验室,长沙410000 [3]岭南现代农业科学与技术广东省实验室,广州510000

出　　处：《畜牧兽医学报》2022年第11期3737-3747,共11页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：湖南省创新平台与人才计划-种业创新项目(2021NK1009);岭南现代农业实验室科研项目(NT2021005)。

摘　　要：旨在研究宁乡猪FABPs、SLC13A5和NR1H4基因的多态性及其与肌内脂肪(IMF)含量的关联性。本试验采用PCR-RFLP和Sanger测序的方法对172头宁乡阉公猪FABPs、SLC13A5和NR1H4基因的多态性进行检测,并探究多态性与宁乡猪IMF含量的关联性。结果表明,宁乡猪H-FABP-HinfⅠ酶切位点存在多态性,其中Hh基因型个体的IMF含量最高,与HH、hh基因型个体的IMF含量差异极显著(P<0.01);H-FABP-HaeⅢ酶切只检测到DD和Dd两种基因型,Dd基因型个体的IMF含量极显著高于DD基因型个体(P<0.01);H-FABP基因的HinfⅠ和HaeⅢ多态性位点共形成3种单倍型和5种单倍型组合,其中单倍型组合HD/hd个体具有最佳的IMF含量。H-FABP的Msp I酶切结果及A-FABP基因测序结果显示,宁乡猪不存在多态现象;SLC13A5基因多态性位点g.50705299T/C和NR1H4基因多态性位点g.83607915G/A处不同基因型间IMF含量的差异均不显著(P>0.05)。综上所述,H-FABP基因HinfⅠ和HaeⅢ酶切位点多态性与宁乡猪IMF含量显著关联,其形成的单倍型组合HD/hd的IMF含量最高。以上结果可为宁乡猪IMF含量性状的早期分子选育提供参考。The study aimed to explore the polymorphisms of FABPs,SLC13 A5 and NR1 H4 genes in Ningxiang pigs and their association with intramuscular fat(IMF) content.In the experiment,the polymorphisms of FABPs,SLC13 A5 and NR1 H4 genes in 172 Ningxiang barrows were detected using PCR-RFLP and Sanger sequencing,and the association between them and IMF content was explored.The results showed that the Hinf Ⅰ digestion site of H-FABP gene was polymorphic in Ningxiang pigs,and the IMF content of individuals with Hh genotype was the highest,which was significantly different from that of individuals with HH and hh genotypes(P0.05).In conclusion,the polymorphisms of H-FABP Hinf Ⅰ and Hae Ⅲ sites were significantly associated with IMF content in Ningxiang pigs,and the pigs with haplotype combination HD/hd had the highest IMF content.The above results could provide reference for the early molecular breeding of IMF content trait in Ningxiang pigs.

关 键 词：宁乡猪 FABPs SLC13A5 NR1H4 肌内脂肪 

分 类 号：S828.2[农业科学—畜牧学]