肉用鹌鹑血管活性肠肽Ⅰ型受体基因的多态性与胴体性状的关联分析  

Association between polymorphism of vasoactive intestinal peptide typeⅠreceptor gene and carcass traits in meat quails

作　　者：王新乐 白俊艳[1,2] 李静云 雷莹 李淦 庞有志 董智豪[1] 陈宇 樊红灯 王龙威 陈梦柯 曾凡林 安肖凯 白永振 WANG Xinle;BAI Junyan;LI Jingyun;LEI Ying;LI Gan;PANG Youzhi;DONG Zhihao;CHEN Yu;FAN Hongdeng;WANG Longwei;CHEN Mengke;ZENG Fanlin;AN Xiaokai;BAI Yongzhen(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,Henan,China;Luoyang Key Laboratory of Animal Genetics and Breeding,Luoyang 471023,Henan,China)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471023 [2]洛阳市动物遗传育种重点实验室,河南洛阳471023

出　　处：《浙江农业学报》2022年第11期2379-2385,共7页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31201777)。

摘　　要：为了探讨血管活性肠肽Ⅰ型受体基因(VIPR-1)的多态性与肉用鹌鹑胴体性状的关系,采用PCR-RFLP方法对法国巨型肉用鹌鹑和莎维麦脱肉用鹌鹑2个群体的VIPR-1基因与肉用鹌鹑胴体性状进行关联分析。结果表明,VIPR-1基因外显子4-5的Bsr DⅠ位点在法国巨型肉用鹌鹑和莎维麦脱肉用鹌鹑中检测到3种基因型,即GG、GT、TT基因型,基因型频率分别为:0.408、0.490、0.102;0.222、0.444、0.334。VIPR-1基因外显子6-7的Hpy CH4Ⅳ位点在法国巨型肉用鹌鹑和莎维麦脱肉用鹌鹑中检测到AA、AG、GG这3种基因型,基因型频率分别为:0.020、0.020、0.960;0.056、0.111、0.833。关联分析表明,VIPR-1基因外显子4-5的Bsr DⅠ位点与法国巨型肉用鹌鹑的肝重有显著关联性(P<0.05);VIPR-1基因外显子6-7的Hpy CH4Ⅳ位点与法国巨型肉用鹌鹑的体重、屠宰重、肝重有显著关联性(P<0.05),与莎维麦脱肉用鹌鹑的体重、屠宰重、全净膛重、心重、胸肌重、腿肌重、肝率、胸肌率有显著关联性(P<0.05)。VIPR-1基因可以作为候选基因应用于肉用鹌鹑的分子标记辅助选择。In order to investigate the relationship between the polymorphism of type 1 vasoactive intestinal peptide receptor(VIPR-1)gene and carcass traits of meat quail,the polymorphism of VIPR-1 gene was detected by PCR-RFLP method in two populations,including French giant meat quail and Savimaito meat quail,and the correlation between the polymorphism of VIPR-1 gene and carcass traits of meat quail was analyzed.The results showed that GG,GT and TT genotypes were detected at the Bsr DⅠlocus of exon 4-5 of VIPR-1 gene in French giant meat quail and Savimaito meat quail,the GG,GT and TT genotype frequencies of French giant meat quail and Savimaito meat quail were 0.408,0.490,0.102;0.222,0.444,0.334,respectively.AA,AG and GG genotypes were detected at the Hpy CH4Ⅳlocus of exon 6-7 of VIPR-1 gene in French giant meat quail and Savimaito meat quail,the AA,AG and GG genotype frequencies of French giant meat quail and Savimaito meat quail were 0.020,0.020,0.960;0.056,0.111,0.833,respectively.The results of association analysis showed that the Bsr DⅠlocus in exon 4-5 of VIPR-1 gene was significantly associated with the liver weight of French giant meat quail(P<0.05).The Hpy CH4Ⅳlocus in exon 6-7 of VIPR-1 gene was significantly associated with body weight,slaughter weight and liver weight of French giant meat quail(P<0.05),and with the body weight,slaughter weight,eviscerated weight,heart weight,chest muscle weight,leg muscle weight,liver rate and chest muscle rate of Savimaito meat quail(P<0.05).The VIPR-1 gene can be used as a candidate gene for marker-assisted selection of carcass traits in meat quail.

关 键 词：肉用鹌鹑 血管活性肠肽Ⅰ型受体(VIPR-1)基因 多态性 胴体性状 关联分析 

分 类 号：S837[农业科学—畜牧学]