庚二酸的生物合成及发酵工艺条件优化  

Biosynthesis and fermentation optimization of pimelic acid

作　　者：鲍青青 毛银 李国辉[1,2] 邓禹 BAO Qingqing;MAO Yin;LI Guohui;DENG Yu(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing,Jiangnan University,Wuxi 214122,China;School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与发酵工业》2022年第22期34-39,共6页Food and Fermentation Industries

基　　金：国家自然科学基金(22008088,21877053);中国博士后科学基金资助项目(2020M681485,2021T140277);江苏省博士后科研资助计划(2020Z012)资助。

摘　　要：庚二酸是一种重要的二元羧酸,广泛应用于医药健康及聚合物制造等多种领域。当前,生物法合成庚二酸存在产量较低、宿主种类较少的缺点,限制了其进一步的工业化生产及衍生物开发。为了提高庚二酸的生物法合成能力,该研究首先利用48孔板和摇瓶分别进行初筛和复筛,得到了1株产庚二酸的霍氏肠杆菌Bpa-3,在摇瓶条件下,该菌株可以合成115.4 mg/L的庚二酸。随后对菌株Bpa-3的发酵工艺条件进行了优化,发现当以甘油为碳源,胰蛋白胨为氮源,接种量为4%并外加30 mmol/L KH_(2)PO_(4)时,庚二酸的产量最高,为217.3 mg/L,是优化前的1.89倍,为庚二酸的生物法合成提供了新宿主,也为其工业化开发奠定了基础。Pimelic acid is an important dicarboxylic acid,which is broadly applied in medicine,polymer manufacturing and other industries.Currently,the biosynthesis of pimelic acid has the problems of low yield and few kinds of hosts,which limits its further industrial production and derivatives development.In order to increase the biosynthesis yield of pimelic acid,this study firstly used 48-well plate and shake flasks for primary screening and re-screening,respectively,obtaining a pimelic acid-producing Enterobacter hormaechei Bpa-3,of which the pimelic acid titer was 115.4 mg/L during shake flask fermentation.Subsequently,the fermentation conditions of strain Bpa-3 were optimized.It was found that when the carbon source was glycerol,the nitrogen source was tryptone,the amount of inoculum was 4%and adding 30 mmol/L KH_(2)PO_(4),the titer of pimelic acid was the highest,which was 217.3 mg/L and1.89-fold than that before optimization,providing a new host for the biosynthesis of pimelic acid and laying foundations for its industrial development.

关 键 词：庚二酸 生物合成 高效液相色谱法 霍氏肠杆菌 发酵优化 

分 类 号：TQ921[轻工技术与工程—发酵工程]