构建氯化血红素/G-四链体DNA酶比色生物传感器检测食品中苏丹红  

A Colorimetric Biosensor Based on Hemin/G-quadruplex DNAzymes for the Detection of Sudan Dyes in Foods

作　　者：卢春霞 闫圣坤[2] 刘成江[3] 林祥群[4] 王双慧 冯晓汀 LU Chunxia;YAN Shengkun;LIU Chengjiang;LIN Xiangqun;WANG Shuanghui;FENG Xiaoting(School of Advanced Agriculture and Bioengineering,Yangtze Normal University,Chongqing 408100,China;Institute of Agricultural Mechanization,Xinjiang Academy of Agricultural Sciences,Ürümqi 830091,China;Agricultural Products Processing Research Institute,Academy of Agriculture and Reclamation Science,Shihezi 832000,China;Xinjiang Shihezi Vocational Technical College,Shihezi 832000,China)

机构地区：[1]长江师范学院现代农业与生物工程学院,重庆408100 [2]新疆农业科学院农业机械化研究所,新疆乌鲁木齐830091 [3]新疆农垦科学院农产品加工研究所,新疆石河子832000 [4]新疆石河子职业技术学院,新疆石河子832000

出　　处：《食品科学》2022年第22期346-352,共7页Food Science

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0080);长江师范学院科研资助项目;苏州相城区科技领军人才计划项目。

摘　　要：以苏丹红Ⅲ核酸适配体为识别元件,以氯化血红素/G-四链体DNA酶为信号探针,结合杂交链反应信号放大策略,构建一种简单、新颖、高灵敏的生物传感器,用于苏丹红比色检测。在优化条件下,对该方法的灵敏度、准确性、特异性进行评估,最后将其应用食品中苏丹红Ⅲ快速检测,并与GB/T 19681-2005法对比验证。结果显示,在优化条件下,苏丹红Ⅲ质量浓度在0.5~250 ng/mL范围与450 nm波长处的吸光度呈良好线性关系(相关系数为0.995),方法检测限为0.09 ng/mL。特异性分析显示,本方法可用于苏丹红I~IV的检测。实际样品分析中表明,食品中苏丹红Ⅲ加标回收率为84.3%~101.6%,相对标准偏差为4.13%~8.36%,本方法的加标回收率与GB 19681-2005法相比差异不显著(P>0.05)。该方法操作简便、准确可靠、灵敏度高,适用于批量食品中苏丹红的快速检测。A simple, novel, and sensitive biosensor for the colorimetric detection of Sudan dyes was successfully prepared using Sudan Ⅲ aptamer as a recognition element, hemin/G-quadruplex DNAzyme as a signal indicator, and hybridization chain reaction as a signal amplification strategy. Under optimized conditions, the sensitivity, accuracy and specificity of the method were evaluated. Finally, the developed method was applied to detect Sudan Ⅲ in food samples, and was validated by comparing it with the national standard method(GB/T 19681-2005). A good linear relationship was obtained between the absorbance at 450 nm and the concentration of Sudan Ⅲ in the range of 0.5 to 250 ng/m L with correlation coefficient of 0.995, and the detection limit was 0.09 ng/mL. Specificity analysis demonstrated that this method could be used to detect Sudan I–IV. The recoveries of Sudan Ⅲ from spiked real samples ranged from 84.3% to 101.6%, with relative standard deviations of 4.13%–8.36%. There was no significant difference in the spiked recoveries between the proposed method and the national standard method(P > 0.05). This method was simple, reliable, sensitive, and suitable for the detection of Sudan dyes in food samples.

关 键 词：苏丹红 适配体 杂交链式反应 氯化血红素/G-四链体DNA酶 比色生物传感器 

分 类 号：O657.3[理学—分析化学]