酶促合成己二酸胆固醇基单乙烯基酯的研究  

Study on enzymatic synthesis of(5-cholesten-yl)vinyl adipic acid

作　　者：赵莹[1] 曾佳裕 刘小敏 聂华[1] ZHAO Ying;ZENG Jia-Yu;LIU Xiao-Min;NIE Hua(Medical College of Jiaying University,Meizhou 514031,China;Institute of Hakka Medicinal Biological Resources,Medical College of Jiaying University,Meizhou 514031,China)

机构地区：[1]嘉应学院医学院,广东梅州514031 [2]嘉应学院医学院客家药用生物资源研究所,广东梅州514031

出　　处：《化学研究与应用》2022年第11期2630-2635,共6页Chemical Research and Application

基　　金：广东省自然科学基金-面上项目(2022A1515011529)资助;梅州市应用型科技专项资金项目(2020B0205003)资助;广东省普通高校特色创新项目(2020KTSCX137)资助;广东省医学科研基金项目(A2020372)资助;广东省大学生创新创业训练计划项目(S202010582086)资助。

摘　　要：以胆固醇和己二酸二乙烯酯为原料在脂肪酶PS IM催化剂的作用下合成己二酸胆固醇基单乙烯基酯,并进行了合成工艺单因素优化实验,优化后的工艺条件为:胆固醇20 mg,己二酸二乙烯酯100 mg,4分子筛50 mg,脱水正己烷5 mL,PS IM 5 U,温度45℃,反应时间4 h,在上述反应条件下重复3次实验,己二酸胆固醇基单乙烯基酯的平均产率为99.2%。Using cholesterol and adipic acid divinyl ester as raw materials,lipase PS IM as catalyzer,(5-cholesten-yl)vinyl adipic acid was synthesized,and the synthesis process had been optimized by the single factor method.The optimized process conditions were as followings:cholesterol 20 mg,adipic acid divinyl ester 100 mg,4 molecular sieve 50 mg,dehydrated n-hexane 5 mL,PS IM 5 U,reaction temperature 45℃and reaction time 4 h.The average yield of(5-cholesten-yl)vinyl adipic acid was found to be 99.2%with 3 repeated experiments under the conditions mentioned above.

关 键 词：酶催化 胆固醇 脂质体 靶向递药 

分 类 号：O629.21[理学—有机化学]