铁冬青酸对人肺腺癌细胞增殖、迁移和侵袭能力的影响  

Effect of Rotundic acid on proliferation,migration and invasion of human lung adenocarcinoma cells

作　　者：杨彩梅 束军[1] 郑江霞 沈继龙 Yang Caimei;Shu Jun;Zheng Jiangxia;Shen Jilong(Dept of Respiratory Medicine,The Fourth Affiliated Hospital of Anhui Medical University,Hefei 230011;The Key Laboratory of Microbiology and Parasitology of Anhui Province,the Key Laboratory of Zoonoses of High Institutions in Anhui,Hefei 230032)

机构地区：[1]安徽医科大学第四附属医院呼吸内科,合肥230011 [2]病原生物学安徽省重点实验室,人畜共患病安徽高校省级重点实验室,合肥230032

出　　处：《安徽医科大学学报》2022年第11期1782-1789,共8页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究重点项目(编号:KJ2018A0204)。

摘　　要：目的观察铁冬青酸(RA)对人肺腺癌细胞增殖、迁移和侵袭能力的影响,并初步探讨其机制。方法以人肺腺癌A549和PC9细胞作为研究对象,分为细胞对照组、空白对照组、溶剂组和20、40、60、80μmol/L铁冬青酸实验组。使用CCK-8实验和划痕实验分别检测人肺腺癌细胞的增殖能力和水平迁移能力;用Transwell迁移实验和Transwell侵袭实验分别检测各组A549和PC9细胞的纵向迁移能力和侵袭能力;用ELISA检测各组细胞上清液中酪氨酸蛋白激酶2(JAK2)和转录激活蛋白3(STAT3)的蛋白表达量,用RT-PCR法检测各组细胞中JAK2和STAT3的mRNA表达水平。分析上述各指标的组间差异。结果RA作用于肺腺癌细胞后,与对照组相比,实验组人肺腺癌A549和PC9细胞活力下降(P<0.05),肺腺癌细胞的迁移面积减少(P<0.05),穿过聚碳酸酯膜和基质胶的细胞数量均减少(P<0.05),细胞上清液中JAK2和STAT3蛋白表达量降低(P<0.05),JAK2和STAT3的mRNA表达水平降低(P<0.05),且上述指标的降低呈浓度依赖性,组间差异有统计学意义(P<0.05);对照组与溶剂组人肺腺癌A549和PC9细胞活力组间差异无统计学意义。结论RA在体外实验中对于人肺腺癌A549和PC9细胞的增殖、迁移和侵袭能力具有抑制作用,且呈一定的浓度依赖性;其作用机制可能与细胞JAK2/STAT3通路的负向调控有关。Objective To investigate the effect of Rotundic acid(RA)on proliferation,migration and invasion ability of human lung adenocarcinoma cells as well as its possible mechanisms.Methods Human lung adenocarcinoma A549 and PC9 cells were divided into control group,blank control group,solvent group and 20,40,60,80μmol/L RA groups.CCK-8 assay and scratch assay were used to detect the proliferation and horizontal migration of human lung adenocarcinoma cells.Transwell migration assay and Transwell invasion assay were used to detect the longitudinal migration and invasion ability of A549 and PC9 cells in each group.The protein expression levels of janus kinase 2(JAK2)and signal transducer and activator of transcription 3(STAT3)in the supernatants of A549 and PC9 cells were detected by ELISA.The mRNA expression levels of JAK2 and STAT3 were detected by RT-PCR.Statistical analysis was made on the differences among groups in each index.Results After RA treatment on human lung adenocarcinoma cells,compared with the control group,the proliferation activity of A549 and PC9 cells in the experimental groups decreased(P<0.05),the number of cells crossing polycarbonate membrane and matrix glue decreased(P<0.05),the expression of JAK2 and STAT3 proteins in cell supernatant decreased(P<0.05),and the mRNA expression of JAK2 and STAT3 decreased(P<0.05).The decrease of the above indices was concentration-dependent and had statistical significance(P<0.05).Compared with the control group,the proliferation activity of A549 and PC9 cells in the solvent group showed no significant difference.Conclusion RA may inhibit the proliferation,migration and invasion of human lung adenocarcinoma A549 and PC9 cells in vitro,possibly through the inhibition of JAK2/STAT3 pathway.

关 键 词：铁冬青酸 人肺腺癌细胞 增殖 迁移 侵袭 酪氨酸蛋白激酶2 转录激活蛋白3 

分 类 号：R734.2[医药卫生—肿瘤]