艾叶挥发油成分分析及其抗氧化活性谱效关系  

Spectrum-effect Relationship between Volatile Constituent and Antioxidant Activity of Artemisia argyi

作　　者：李雨铭 李齐激 王丽[2,3] 杨艳[2,3] 葛丽娟 杨小生[1,2,3] LI Yu-ming;LI Qi-ji;WANG Li;YANG Yan;GE Li-juan;YANG Xiao-sheng(Guizhou University of Traditional Chinese Medicine,Guizhou Guiyang 550025;Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guizhou Guiyang 550014;Engineering Research Center for Efficient Utilization of Natural Products in Guizhou Province,Guizhou Guiyang 550014,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014 [3]贵州省天然产物高效利用工程研究中心,贵州贵阳550014

出　　处：《广州化工》2022年第20期121-124,共4页GuangZhou Chemical Industry

基　　金：贵州省科技支撑项目(黔科合支撑2022一般134);贵州省科技创新能力建设专项(黔科合服企[2020]4013);贵州省发展和改革委员会(黔财建[2019]303);贵州省教育厅高等学校特色重点实验室(黔教合KY字[2020]018)。

摘　　要：利用多元统计分析构建气相色谱-质谱联用(GC-MS)数据与抗氧化活性的相关性,进而阐明艾叶挥发油抗氧化活性的主要成分类群。采用GC-MS法结合三种抗氧化活性方法,利用灰色关联度和聚类分析法分析GC-MS色谱图与抗氧化活性的谱效关系。结果表明,10产地艾叶挥发油组分相对含量介于73.906%~92.234%间。DPPH自由基IC50值、ABTS+与FRAP均值分别介于0.762~3.080 mg/mL、0.3972~0.9688 mmol/g、0.1308~0.2597 mmol/L。基于谱效关系分析,单萜、倍半萜是艾叶挥发油抗氧化活性的主要贡献者,关联度最高为0.934。The correlation between gas chromatography-mass spectrometry(GC-MS)data and antioxidant activity was established by using multivariate statistical analysis,which further clarifyied the main antioxidant active constituents of volatile oils from Artemisia argyi.GC-MS method combined with three antioxidant activity methods was adopted,the spectrum-effect relationship between GC-MS chromatogram and antioxidant activity was established by grey correlation degree and cluster analysis.The results showed that the relative contents of volatile oils were 73.906%~92.234%in 10 habitats.IC_(50) values of DPPH radical,the mean values of ABTS+and total antioxidant capacity assay kit were 0.762~3.080 mg/mL,0.3972~0.9688 mmol/g and 0.1308~0.2597 mmol/L,respectively.The results showed that monoterpenes and sesquiterpenes were the main contributors to the antioxidant activity of the volatile oils from Artemisia argyi leaves,and the highest correlation coefficient was 0.934.

关 键 词：艾叶 挥发油 GC-MS法 抗氧化活性 谱效关系 

分 类 号：R284.1[医药卫生—中药学]