C型凝集素结构域家族7成员A在胶质瘤的意义  

Significance of C-type lectin domain containing 7A in gliomas

作　　者：张春雨 周伟 苏忠周[2] 叶立果 田道锋[1] Zhang Chunyu;Zhou Wei;Su Zhongzhou;Ye Liguo;Tian Daofeng(Department of Neurosurgery,Renmin Hospital of Wuhan University,Wuhan 430060,China;Department of Neurosurgery,Huzhou Central Hospital,Huzhou 313099,China;Department of Anesthesiology,Huzhou Central Hospital,Huzhou 313099,China)

机构地区：[1]武汉大学人民医院神经外科,430060 [2]湖州市中心医院神经外科,313099 [3]湖州市中心医院麻醉科,313099

出　　处：《中华实验外科杂志》2022年第9期1648-1650,共3页Chinese Journal of Experimental Surgery

摘　　要：目的C型凝集素结构域家族7成员A(CLEC7A)在脑胶质瘤中的表达及临床意义。方法利用基因表达谱数据交互分析(GEPIA)及GlioVis数据库分析CLEC7A在胶质瘤组织中表达水平。通过癌症基因组图谱(TCGA)及中国脑胶质瘤基因图谱计划(CGGA)数据库共收集1095例胶质瘤样本,用于分析CLEC7A表达与胶质瘤临床病理特征及预后相关性。使用富集分析明确CLEC7A参与的生物学过程。利用单细胞转录组测序数据分析及Spearman相关检验分析CLEC7A表达与胶质瘤免疫浸润的相关性。结果与非肿瘤脑组织相比(Gravendeel:4.247±0.121;Rembrant:5.972±0.400),CLEC7A在胶质瘤组织中显著上调(Gravendeel:5.134±0.577;Rembrant:6.399±0.676),差异有统计学意义(Gravendeel:t=-4.333,P<0.01;Rembrant:t=-3.292,P<0.01)。多因素Cox回归分析显示CLEC7A表达水平是胶质瘤患者预后的独立影响因素[TCGA:风险比(HR):1.637;CGGA:HR:1.678,均P<0.05]。GeneMANIA数据库分析显示CLEC7A与半乳糖凝集素-3(LGALS3)具有强相关性。利用单细胞转录组测序数据(GSE117891)及肿瘤免疫单细胞中心(TISCH)网站分析显示CLEC7A在胶质瘤免疫微环境中胶质瘤相关性小胶质细胞/巨噬细胞(GAMMs)中特异性高表达,并且利用Spearman检验基于TIMER数据进行验证(低级别胶质瘤:R=0.749;高级别胶质瘤:R=0.205,均P<0.01)。结论CLEC7A可能是胶质瘤预后评估及精准治疗的新型标志物。Objective To investigate the expression and clinical significance of C-type lectin domain containing 7A(CLEC7A)in glioma.Methods The expression level of CLEC7A in gliomas was analyzed using Gene Expression Profiling Interactive Analysis(GEPIA)and GlioVis databases.Totally,1095 glioma samples were collected from The Cancer Genome Atlas(TCGA)and Chinese Glioma Genome Atlas(CGGA)databases.The correlation between CLEC7A expression and clinicopathological features and survival of gliomas was analyzed.Enrichment analysis was used to explore the biological processes.The correlation between CLEC7A expression in glioma and immune infiltration was analyzed by single-cell RNA sequencing(scRNA-seq)analysis and TIMER online website.Results Compared with non-tumor brain tissues(Gravendeel:4.247±0.121;Rembrant:5.972±0.400),CLEC7A was significantly up-regulated in glioma tissues(Gravendeel:5.134±0.577;Rembrant:6.399±0.676),and the differenceswere statistically significant(Gravendeel:t=-4.333,P<0.01;Rembrant:t=-3.292,P<0.01).Multivariate Cox regression analysis showed that CLEC7A expression level was an independent prognostic factor in gliomas[TCGA:hazard ratio(HR):1.637;CGGA:HR:1.678,P<0.05,respectively].GeneMANIA database analysis demonstrated that CLEC7A was strongly associated with LGALS3(galectin 3).The scRNA-seq data(GSE117891)and online analysis through Tumor Immune Single-cell Hub(TISCH)website revealed that CLEC7A was specifically upregulated in glioma-associated microglia/macrophages(GAMMs)in glioma immune microenvironment(GIME),which was verified in the TIMER data using Spearman test[low-grade glioma(LGG):R=0.749;high-grade glioma(HGG):R=0.205,P<0.01).Conclusion CLEC7A might be a novel marker for glioma prognosis assessment and precise therapy.

关 键 词：C型凝集素结构域家族7成员A 单细胞转录组测序 肿瘤免疫微环境 胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]