神经轴突导向因子2对神经胶质瘤细胞的增殖、迁移和糖酵解的影响  

Effects of axonal guidance factor 2 on proliferation,migration and glycolysis of glioma cells

作　　者：张卫平[1] 温一阳[2] 孙永臣[3] 王晓朦 屠淑敏[1] 李依明[4] Zhang Weiping;Wen Yiyang;Sun Yongchen;Wang Xiaomeng;Tu Shumin;Li Yiming(Emergency Department of the First People’s Hospital of Shangqiu City,Shangqiu 476100,China;Oncology Center of Henan Provincial People’s Hospital,Zhengzhou 450003,China;Department of Radiotherapy,the First People’s Hospital of Shangqiu City,Shangqiu 476100,China;The Imaging Center of the First People’s Hospital of Shangqiu City,Shangqiu 476100,China)

机构地区：[1]河南省商丘市第一人民医院急诊科,476100 [2]河南省人民医院肿瘤中心,郑州450003 [3]河南省商丘市第一人民医院放疗科,476100 [4]河南省商丘市第一人民医院影像中心,476100

出　　处：《中华实验外科杂志》2022年第9期1655-1657,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨神经轴突导向因子2(Slit2)对神经胶质瘤细胞的增殖、迁移和糖酵解的影响。方法选取2018年6月到2022年6月河南省商丘市第一人民医院收治的61例神经胶质瘤和癌旁组织作为研究对象,采用免疫组织化学分析Slit2蛋白在神经胶质瘤和癌旁组织的阳性率。将神经胶质瘤细胞株U251堆积分为对照组和Slit2 KD组,分别采用细胞计数试剂盒(CCK-8)和体外移植瘤实验分析细胞增殖;采用Transwell和划痕实验分析细胞迁移;采用试剂盒检测葡萄糖提呈和乳酸水平;采用生物化学酶法检测己糖激酶(HK)、丙酮酸激酶(PK)和乳酸脱氢酶(LDH)的活性。组间比较采用t检验。结果神经胶质瘤组织中Slit2蛋白表达阳性率(43/61,72.13%)明显低于癌旁组织(16/61,26.23%),差异有统计学意义(χ2=12.091,P<0.05)。对照组细胞活力(1.86±0.07)明显高于Slit2 KD组细胞(1.22±0.08),差异有统计学意义(t=13.910,P<0.05)。对照组细胞在体内成瘤体积[(829.50±44.12)mm3]明显高于Slit2 KD组细胞[(531.00±64.42)mm3],差异有统计学意义(t=12.090,P<0.05)。对照组细胞在体内成瘤重量[(4.63±0.66)g]明显高于Slit2 KD组细胞[(2.22±0.20)g],差异有统计学意义(t=11.070,P<0.05)。对照组细胞迁移数量[(81.17±4.49)个]和划痕愈合率[(78.33±8.04)%]明显高于Slit2 KD组细胞(56.50±6.66)[(54.67±6.21)%],差异有统计学意义(t=7.525、5.703,P<0.05)。对照组细胞己糖激酶(HK)、丙酮酸激酶(PK)和乳酸脱氢酶(LDH)活性(0.96±0.04、0.95±0.04、0.97±0.04)明显高于Slit2 KD组细胞(0.64±0.09、0.73±0.04、0.51±0.05),差异有统计学意义(t=7.961、8.963、15.780,P<0.05)。对照组细胞葡萄糖摄取量和乳酸水平[(0.75±0.08)、(3.92±0.31)mmol/L]明显高于Slit2 KD组细胞[(0.32±0.04)、(2.75±0.28)mmol/L],差异有统计学意义(t=12.350、6.782,P<0.05)。结论Slit2在神经胶质瘤细胞中高表达,通过调节糖酵解通路关键酶活,介导神经胶质瘤细胞的增殖和迁Objective To investigate the effects of axon guidance factor 2(Slit2)on the proliferation,migration and glycolysis of glioma cells.Methods Totally,61 cases of gliomas and adjacent tissues treated in our hospital from June 2018 to June 2022 were selected as the research objects.The positive rate of Slit2 protein in gliomas and adjacent tissues was analyzed by immunohistochemistry.The glioma cell line U251 was divided into control group and Slit2 KD group,and cell proliferation was analyzed by cell counting kit-8(CCK-8)assay and in vitro tumor transplantation experiment respectively.Transwell assay and scratch test were used to analyze cell migration.Glucose presentation and lactate levels were detected by kit.The activities of hexokinase(HK),pyruvate kinase(PK)and lactate dehydrogenase(LDH)were detected by biochemical enzyme method.T-test was used to compare the measurement data between groups.Results The positive rate of Slit2 protein expression in glioma tissues(43/61,72.13%)was significantly lower than that in adjacent tissues(16/61,26.23%,χ2=12.091,P<0.05).The cell viability of the control group(1.86±0.07)was significantly higher than that of the Slit2 KD group(1.22±0.08,t=13.910,P<0.05).The tumorigenic volume of cells in the control group[(829.50±44.12)mm3]was significantly greater than that in the Slit2 KD group[(531.00±64.42)mm3,t=12.090,P<0.05].The tumor weight of cells in the control group[(4.63±0.66)g]was significantly greater than that of cells in Slit2 KD group[(2.22±0.20)g,t=11.070,P<0.05].The number of migrating cells[(81.17±4.49)]and the wound healing rate[(78.33±8.04)%]in the control group were significantly higher than those in the Slit2 KD group[(56.50±6.66),(54.67±6.21)%,t=7.525,5.703,P<0.05].The activities of HK,PK and LDH in the control group(0.96±0.04,0.95±0.04,0.97±0.04)were significantly higher than those in the Slit2 KD group(0.64±0.09,0.73±0.04,0.51±0.05,t=7.961,8.963,15.780,P<0.05).The glucose intake and lactic acid level of cells in the control group[(0.75±0.08),(3.92�

关 键 词：神经轴突导向因子2 胶质瘤 增殖 迁移 糖酵解 

分 类 号：R739.4[医药卫生—肿瘤]