飞秒激光消蚀血栓动态机制数值仿真和体外生物学验证研究  

Study on Numerical Simulation of Dynamic Mechanism of Thrombus Ablation with Femtosecond Laser & In Vitro Biological Verification

作　　者：刘骁征 李有楠 张海涛[3] 顾瑛[1,4,5] 吴巍巍[2] 张童[2] 刘慕涛 LIU Xiaozheng;LI Younan;ZHANG Haitao;GU Ying;WU Weiwei;ZHANG Tong;LIU Mutao(School of Optics and Photonics,Beijing Institute of Technology,Beijing 100081,China;Department of Vascular Surgery,Beijing Tsinghua Changgung Hospital,School of Clinical Medicine,Tsinghua University;Key Laboratory of Photonic Control Technology,Ministry of Education,Center for Photonics and Electronics,Department of Precision Instrument,Tsinghua University;Department of Laser Medicine,the First Medical Center of PLA General Hospital;Institute of Engineering Medicine,Beijing Institute of Technology;China Electronic Greatwall Shengfeifan Information System CO.,LTD)

机构地区：[1]北京理工大学光电学院,北京市100081 [2]清华大学附属北京清华长庚医院血管外科清华大学临床医学院 [3]清华大学精密仪器系激光与光子技术研究室光子测控技术教育部重点实验室 [4]解放军总医院第一医学中心激光医学科 [5]北京理工大学医学技术学院 [6]中电长城圣非凡信息系统有限公司

出　　处：《中国激光医学杂志》2022年第5期241-248,297-299,共11页Chinese Journal of Laser Medicine & Surgery

基　　金：中国医学科学院医学与健康科技创新工程(2019-I2M-5-061)。

摘　　要：目的观察飞秒激光在体外消蚀血栓的作用。方法通过构建一种等离子体-温度时空演化模型,采用变系数有限差分求解方法,分析单脉冲和多脉冲作用时,血栓在飞秒激光照射过程中物理性质的动态变化以及等离子体介导作用,并结合体外消蚀实验进行验证。结果多脉冲照射可显著提升消蚀效果,根据激光参数与等离子体密度、血栓温度、消蚀效果间的关系分析获得的理想血栓消蚀效果的激光参数范围和照射方式为:脉宽250 fs、脉冲能量10μJ、脉冲个数10个、光斑直径100μm;在多脉冲序列照射实验中,样本未出现烧焦现象,消蚀边缘锐利。结论多脉冲飞秒激光消蚀血栓具有安全性和高效性,可为飞秒激光的血栓清除应用开发提供理论参考。Objective Femtosecond laser has the potential to become a new thrombectomy technology,This paper aims at investigating the mechanism of thrombus ablation in vitro with femtosecond laser.Methods A plasma temperature spatio-temporal evolution model was proposed.The dynamic changes to the physical properties of thrombi in the process of irradiation with femtosecond laser and the plasma mediated effect were considered.Variable coefficient finite difference solution method was used to analyze the action of single-pulse and multiple-pulse respectively.The results were verified via in vitro experiments.Results It is found in this study that multiple-pulse irradiation significantly improved the ablation effect.The range of laser parameters and irradiation mode of ideal thrombus ablation effect were obtained according to the relationship between laser parameters and plasma density,thrombus temperature and ablation effect.The safety and effectiveness of multiple-pulse ablation were verified with in vitro experiments.Conclusions Multiple-pulse femtosecond laser ablation of thrombus is safe and efficient.It provides theoretical reference for the application and development of femtosecond laser thrombus removal equipment.

关 键 词：飞秒激光 生物材料 组织消蚀 等离子体 数值分析 

分 类 号：TN249[电子电信—物理电子学] O242.1[理学—计算数学] R454.2[理学—数学]