Cystatin 6蛋白在哈萨克族食管鳞癌组织中的表达及其意义  

Clinicopathological significance of cystatin 6 protein expression in Kazakh esophageal squamous cell carcinoma

作　　者：李子垚 张海平[1] 买地尼也提·尼亚孜 居来提·艾尼瓦尔[1] 张力为[1] 伊地力斯·阿吾提[1,2] LI Ziyao;ZHANG Haiping;MADINIYET·Niyaz;JULATI·Ainiwaer;ZHANG Liwei;EDRIS·Awut(Department of Thoracic Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054;State Key Laboratory of Causes and Prevention of High Incidence in Central Asia,Clinical Medicine Research Institute,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang,China)

机构地区：[1]新疆医科大学第一附属医院胸外科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院临床医学研究院省部共建中亚高发病成因与防治国家重点实验室,新疆乌鲁木齐830054

出　　处：《癌变．畸变．突变》2022年第6期429-433,438,共6页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(81960498);国家重点实验室开放课题(SKL-HIDCA-2021-21)。

摘　　要：目的:检测哈萨克族食管鳞癌患者的癌组织及其癌旁正常组织人型半胱氨酸蛋白酶抑制剂cystatin 6(cystatin M/E,CST6)的蛋白表达水平,并分析其与临床病理指标和预后之间的关系。方法:收集2010年12月—2020年12月行食管癌根治术的55例哈萨克族食管鳞癌患者癌组织及癌旁正常组织标本,采用免疫组织化学染色法检测其CST6蛋白的表达情况。χ^(2)检验分析CST6蛋白表达和患者临床病理指标之间的关系,Kaplan-Meier单因素和COX多因素分析影响食管癌预后的危险因素。结果:CST6蛋白的表达主要定位于细胞质、细胞膜以及角化区。食管鳞癌组织中CST6蛋白的阳性表达率为36.4%(20/55),癌旁正常组织中的阳性表达率为63.6%(35/55),差异具有统计学意义(χ^(2)=9.032,P=0.003)。CST6蛋白的表达水平与家族史(P=0.045)和肿瘤分化程度(P=0.036)有关,无肿瘤家族史以及肿瘤高分化的患者其阳性表达率更高。Kaplan-Meier生存分析发现,CST6蛋白的表达水平与患者的总生存率有关(P<0.05)。结论:CST6蛋白在哈萨克族食管鳞癌患者中呈低表达,CST6的低表达可能是哈萨克族食管鳞癌发病及不良预后的危险因素之一。OBJECTIVE:To study expression of the cystatin 6(CST6)protein in esophageal squamous cell carcinomas among Kazakhs,and to explore its relationship with clinicopathological parameters and prognosis.METHODS:From December 2010 to December 2020,tissues from 55 Kazakh patients who had esophagectomy at the First Affiliated Hospital of Xinjiang Medical University were collected.Expression of CST6 in cancer and corresponding paracancerous tissues was detected by immunohistochemical method.Relationships between its expression and clinicopathological features was analyzed byχ^(2) test,Kaplan-Meier univariate analysis and COX multivariate analysis to evaluate their relationships with prognosis of esophageal cancer.RESULTS:Expression of the CST6 protein was mainly located in the cytoplasm,cell membrane and keratinized area.The levels of expression of CST6 were 36.4%in the cancer and 63.6%in the corresponding paracancerous tissues.The difference was statistically significant(χ^(2)=9.032,P=0.003).CST6 expression was related to family history(P=0.045)and tumor differentiation(P=0.036):higher in patients with no family history of tumor and with well-differentiated tumors.Kaplan-Meier survival analysis showed that the expression was related to the survival rates of patients(P<0.05):the average survival time of patients with high CST6 expression was longer.Cox multivariant survival analysis showed that negative expression was an independent risk factor for poor prognosis.CONCLUSION:CST6 protein was significantly under-expressed in esophageal squamous cell carcinoma among Kazakh patients.The protein therefore may play a tumor suppression role and its negative expression was a risk factor for poor prognosis.

关 键 词：哈萨克族 食管鳞癌 半胱氨酸组织蛋白酶抑制剂 抑癌基因 预后 

分 类 号：R735.1[医药卫生—肿瘤]