PTEN C末端对鼻咽癌细胞迁移及增殖能力的影响  

Effect of PTEN C-tail on migration and proliferation of nasopharyngeal carcinoma cells

作　　者：张军军 梁乐平 ZHANG Junjun;LIANG Leping(Department of Otolaryngology-head and Neck Surgery,Tangdu Hospital,Air Force Medical University,Xi'an 710038,Shaanxi,China)

机构地区：[1]空军军医大学唐都医院耳鼻喉科,陕西西安710038

出　　处：《癌变．畸变．突变》2022年第6期434-438,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究PTEN C末端对鼻咽癌细胞迁移及增殖能力的影响。方法:采用荧光定量PCR实验检测PTEN mRNA在鼻咽癌细胞株HONE1、SUNE1、CNE1、CNE2、5-8F和鼻咽上皮细胞NP69中的表达情况。选择5-8F和HONE1细胞,用含有TGF-β1(5 ng/mL)的RPMI 1640培养基进行细胞培养,分别瞬时转染阴性对照(NC)、PTEN WT(野生型)和缺乏C末端结构的PTEN 1-353(突变体)质粒,并采用Western blot实验验证转染效率。转染成功后48 h,分别采用Transwell实验、CCK-8法细胞增殖实验检测PTEN WT和PTEN 1-353对鼻咽癌细胞株5-8F和HONE1细胞迁移、增殖的影响。结果:与NP69细胞相比,PTEN mRNA在鼻咽癌细胞中的表达水平明显降低(P<0.01)。与转染NC组比较,PTEN WT和PTEN 1-353均可以抑制TGF-β1诱导的鼻咽癌细胞的迁移(P<0.01),但此二组之间差异无统计学意义(P>0.05);PTEN WT和PTEN 1-353均可抑制TGF-β1诱导的鼻咽癌细胞增殖(P<0.05),且相比于PTEN WT,PTEN 1-353对鼻咽癌细胞增殖的抑制能力降低(P<0.01)。结论:PTEN C末端未参与鼻咽癌细胞的迁移过程,但可能与鼻咽癌细胞的增殖有关。OBJECTIVE:To elucidate functions of the PTEN C-tail in nasopharyngeal carcinoma(NPC).METHODS:Fluorescence quantitative PCR was used to detect expressions of PTEN in NPC cell lines and nasopharyngeal epithelial cell lines,NP69.5-8F and HONE1.Cells were cultured with 5 ng/mL TGF-β1.Negative control(NC),PTEN WT(wild-type)and PTEN 1-353(lacking C-tail structure of PTEN)plasmids were over-expressed in the cells.Western blot were used to verify their transient efficiency.After 48 h,effects of PTEN WT and PTEN 1-353 on metastasis abilities were verified by Transwell experiments.Effect of PTEN WT and PTEN 1-353 on proliferation capacities were verified by CCK-8 assays.RESULTS:Compared with NP69,expressions of PTEN in NPC cells were reduced.Compared with the NC group,the migration abilities of TGF-β1-induced NPC cells were inhibited by PTEN WT and PTEN 1-353(P<0.01),but there was no statistical difference between PTEN WT and PTEN 1-353(P>0.05).Compared with the NC group,the proliferation abilities of TGF-β1-induced NPC cells were inhibited by PTEN WT and PTEN 1-353(P<0.05).Interestingly,the inhibition of PTEN 1-353 was weaker than PTEN WT(P<0.05).CONCLUSION:The PTEN C-tail was not involved in the regulation of metastasis of NPC cells.However,the PTEN C-tail could be involved in regulating the proliferation ability of NPC cells.

关 键 词：PTEN C末端 细胞迁移 细胞增殖 鼻咽癌 

分 类 号：R739.6[医药卫生—肿瘤] R730.2[医药卫生—临床医学]