人参培养须根的大鼠致畸试验  

Study on teratogenicity of cultured ginseng fibrous root in rats

作　　者：刘欢[1] 梁菲 李莹[2] 吴莎莎 曹璨 徐建文 刘影[2] LIU Huan;LIANG Fei;LI Ying;WU Shasha;CAO Can;XU Jianwen;LIU Ying(Department of Neurology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001;Food and Drug Safety Evaluation Center of Harbin Medical University,Harbin 150001,Heilongjiang,China)

机构地区：[1]哈尔滨医科大学附属第四医院神经内科,黑龙江哈尔滨150001 [2]哈尔滨医科大学食品药品安全评价中心,黑龙江哈尔滨150001

出　　处：《癌变．畸变．突变》2022年第6期459-462,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：黑龙江省应用技术研究与开发计划重大项目(GA16C106)。

摘　　要：目的:评价人参培养须根的致畸性。方法:将80只雌性和40只雄性SD大鼠,以雌雄2∶1比例合笼。将检出的受孕大鼠按体质量随机分为低、中、高剂量组(分别为2、4、8 g/kg,相当于人体推荐量的60.5、121、242倍)和对照组,每组16只。于受孕6~15 d连续给予受试物,并在第20天处死。剖腹取出子宫称取质量,检查着床数、吸收胎、早死胎、晚死胎及活胎数有无异常,测量胎鼠身长并称取胎仔体质量;对部分胎仔进行外观检查、骨骼检查及内脏检查。结果:与对照组相比,人参培养须根各剂量组孕鼠体质量增量、子宫质量、窝平均活胎数、胎鼠身长、体质量的差异均无统计学意义(P>0.05);对胎鼠进行外观、脏器及骨骼检查,各剂量组和阴性对照组均未见明显异常和畸形。结论:在本实验室条件下,人参培养须根无致畸作用。OBJECTIVE:To evaluate teratogenicity of cultured ginseng fibrous roots in rats.METHODS:80 female and 40 male SD rats were caged in a ratio of 2:1 for mating.Pregnant rats were randomly divided into high-,medium-,and low-dose groups(2,4,8 g/kg,respectively,equivalent to the recommended amount of 60.5,121,242 times according to body mass)and control group,with 16 in each group.The rats were continuously given ginseng between 6-15 days of conception,and were executed on the 20th day.Caesarean sections were conducted to remove the uterus to inspect the quality,and to check the number of implantations,absorption of fetuses,early stillbirth,late stillbirth,and the numbers and abnormalities of live fetuses.Some fetal pups were examined for appearance,and bone and visceral abnormalities.RESULTS:Compared with the control group,there were no significant differences in the body mass,uterine quality,average number of live births,and length and body quality of pregnant rats.The appearance,organs and bones of the fetal rats were examined.Each dose group,there was no obvious abnormalities and deformities in the negative control group.CONCLUSION:The cultured fibrous roots of ginseng showed no teratogenic effects under the conditions of our investigation.

关 键 词：人参 人参培养须根 致畸性 安全性评价 

分 类 号：R394.6[医药卫生—医学遗传学]