板栗壳提取物对SD大鼠的致畸毒性研究  

Teratogenicity study of chestnut shell extract in Sprague-Dawley rats

作　　者：江丹 伍淑文 陈志元 田辉[3] 孙代华 樊柏林[3] JIANG Dan;WU Shuwen;CHEN Zhiyuan;TIAN Hui;SUN Daihua;FAN Bolin(Jing Brand Chizhengtang Pharmaceutical Co.,Ltd.,Huangshi 435100;Yangxin County Center for Disease Control and Prevention,Huangshi 435200;Hubei Provincial Key Laboratory for Applied Toxicology,Hubei Provincial Center for Disease Control and Prevention,Wuhan 430079,Hubei,China)

机构地区：[1]劲牌持正堂药业有限公司,湖北黄石435100 [2]阳新县疾病预防控制中心,湖北黄石435200 [3]湖北省疾病预防控制中心应用毒理湖北省重点实验室,湖北武汉430079

出　　处：《癌变．畸变．突变》2022年第6期463-466,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：湖北省重点研发计划项目(2020ACA007-03)。

摘　　要：目的:研究板栗壳提取物对SD大鼠的致畸毒性。方法:将受孕SD大鼠随机分为低、中、高3个剂量板栗壳提取物组,阴性对照组和阳性对照组,每组18~20只。低、中、高剂量组分别按0.37、1.11和3.33 g/kg给予板栗壳提取物;阴性对照组给予蒸馏水;阳性对照组按13 mg/kg给予维生素A。于妊娠期的第6~15天灌胃给予受试物或对照物,第20天麻醉后处死孕鼠。观察母鼠和胎鼠的生长发育、受孕率、生殖能力、外观、内脏和骨骼畸形等指标。结果:与阴性对照组比较,阳性对照组第9天至第20天孕鼠体质量偏低,体质量增量降低,胎鼠体质量减轻、身长明显降低,其胎鼠外观畸形率、外观畸形窝数率、内脏畸形率、内脏畸形窝数率、骨骼畸形率、畸形窝数率明显升高,差异有统计学意义(P<0.05),表明阳性对照维生素A对大鼠致畸作用明显,模型成功建立。与阴性对照组比较,板栗壳提取物各剂量未观察到对母鼠及胎鼠的毒性和生殖力的影响,未观察到外观、内脏和骨骼畸形,差异均无统计学差异(P>0.05)。结论:在本试验条件下,板栗壳提取物对大鼠母体毒性和胚胎发育毒性未观察到有害作用剂量(NOAEL)大于3.33 g/kg,对大鼠无致畸作用。OBJECTIVE:To study teratogenic effects of chestnut shell extract in Sprague-Dawley(SD)rats.METHODS:Pregnant SD rats were randomly divided into five groups:low,middle and high dosages of chestnut shell extract groups,negative control group and positive control group.The negative control group was given distilled water and the positive control group was given 13 mg/kg vitamin A.The extracts were given intra-gastrically at the 6th to 15th day of gestation,and the pregnant rats were killed at the 20th day of gestation.RESULTS:Compared with the negative control group,the positive control group showed reduced body weight,weight gain and net weight gain from the 9th to the 20th day.In the positive control group,the body weight and body length were obviously decreased,and the rate of deformity,of viscera malformation,and of skeleton malformation were obviously increased.The positive control group showed obvious teratogenic effects.However,there was no significant difference between each dose group and the negative control group.CONCLUSION:Under our experimental conditions,the no observed adverse effect level(NOAEL)of chestnut shell extract on female rats and fetus was above 3.33 g/kg.

关 键 词：板栗壳提取物 致畸毒性 SD大鼠 致畸试验 

分 类 号：R394.6[医药卫生—医学遗传学]