特应性皮炎患儿外周血miR-155表达与Th1/Th17细胞及相关细胞因子的相关性分析  

Correlation analysis of peripheral blood miR-155 expression with Th1/Th17 cells and related cytokines in children with atopic dermatitis

作　　者：周晓宇 罗亭 王润超 ZHOU Xiaoyu;LUO Ting;WANG Runchao(Department of Dermatology,Taihe Hospital,Hubei Province,Shiyan 442000,China)

机构地区：[1]湖北省十堰市太和医院皮肤科,湖北十堰442000

出　　处：《中国医药导报》2022年第32期88-91,共4页China Medical Herald

基　　金：湖北省卫生计生委科研项目(MJ2017Y102);湖北省十堰市太和医院院级项目(2020JJXM006)。

摘　　要：目的探讨特应性皮炎(AD)患儿外周血微RNA(miR)-155表达与辅助性T细胞(Th)1/Th17细胞及相关细胞因子的相关性。方法选择2018年2月至2021年9月湖北省十堰市太和医院(以下简称“我院”)收治的89例AD患儿为AD组,根据特应性皮炎评分指数分为轻度组(28例)、中度组(37例)、重度组(24例),另选择同期我院体检的25名健康儿童为对照组。比较各组外周血miR-155、Th1/Th17细胞及相关细胞因子水平,分析AD患儿外周血miR-155表达与Th1、Th17及相关细胞因子水平的相关性。结果AD组miR-155表达、Th17细胞占比、白细胞介素(IL)-17、IL-23高于对照组,Th1细胞占比、Th1/Th17,γ干扰素(IFN-γ)、IL-2、IFN-γ/IL-17低于对照组(P<0.05)。重度组miR-155表达,Th17细胞占比、IL-17、IL-23高于轻度组和中度组,Th1细胞占比、Th1/Th17、IFN-γ、IL-2、IFN-γ/IL-17低于轻度组和中度组;中度组miR-155表达,Th17细胞占比、IL-17、IL-23高于轻度组,Th1细胞占比、Th1/Th17、IFN-γ、IL-2、IFN-γ/IL-17低于轻度组(P<0.05)。AD患儿miR-155表达与Th17细胞占比、IL-17、IL-23呈正相关(r>0,P<0.05),与Th1细胞占比、Th1/Th17、IFN-γ、IL-2、IFN-γ/IL-17呈负相关(r<0,P<0.05)。结论AD患儿外周血miR-155表达增高,且与Th1/Th17失衡有关,miR-155可能通过调节Th1/Th17参与AD发病过程。Objective To investigate the correlation of peripheral blood microRNA(miR-155)expression with T helper(Th)1/Th17 cells and to related cytokines in children with atopic dermatitis(AD).Methods A total of 89 AD children admitted to Taihe Hospital,Hubei Province(hereinafter referred to as“our hospital”)from February 2018 to September 2021 were selected as AD group,and according to the score of scoring atopic dermatitis,they were divided into mild group(28 cases),moderate group(37 cases),and severe group(24 cases).Another 25 healthy children who underwent physical examination during the same period were selected as control group.The levels of miR-155 in peripheral blood,Th1/Th17 cells,and related cytokines of each group were compared,and the correlation between the expression of miR-155 in peripheral blood and Th1,Th17,and related cytokine levels in children with AD were analyzed.Results The expression of miR-155,the proportion of Th17 cell,interleukin(IL)-17,and IL-23 in AD group were higher than those in control group,and the proportion of Th1 cell,Th1/Th17,interferon-γ(IFN-γ),IL-2,and IFN-γ/IL-17 were lower than those in control group(P<0.05).The expression of miR-155,the proportion of Th17 cell,IL-17,and IL-23 in severe group were higher than those in mild group and moderate group,while the proportion of Th1 cell,Th1/Th17,IFN-γ,IL-2,and IFN-γ/IL-17 were lower than those in mild group and moderate group(P<0.05).The expression of miR-155,the proportion of Th17 cell,IL-17,and IL-23 in moderate group were higher than those in mild group,and the proportion of Th1 cell,Th1/Th17,IFN-γ,IL-2,and IFN-γ/IL-17 in moderate group were lower than those in mild group(P<0.05).The expression of miR-155 in peripheral blood of AD children was positively correlated with the proportion of Th17 cell,serum IL-17 and IL-23(r>0,P<0.05),and negatively correlated with the proportion of Th1 cell,Th1/Th17,IFN-γ,IL-2,and IFN-γ/IL-17(r<0,P<0.05).Conclusion The expression of miR-155 in peripheral blood of AD children is increased,and

关 键 词：特应性皮炎 微RNA 辅助性T细胞 

分 类 号：R000[医药卫生]