伏立诺他协同NVP-BEZ235诱导人外周血白血病T细胞凋亡探究  

Induction of Apoptosis in Human Peripheral Blood Leukemia T Cells by Vorinostat Combined with NVP-BEZ235

作　　者：陈明 肖红[1] Chen Ming;Xiao Hong(Department of Hematology,Xiangtan First People’s Hospital in Hunan Province,Xiangtan 411101,China)

机构地区：[1]湖南省湘潭市第一人民医院血液科,湖南湘潭411101

出　　处：《黑龙江科学》2022年第22期12-14,共3页Heilongjiang Science

摘　　要：为探讨伏立诺他联合NVP-BEZ235诱导人外周血白血病T细胞(Jurkat细胞)凋亡及机制,培养Jurkat细胞,用伏立诺他或(和)NVP-BEZ235处理,采用流式细胞术检测Jurkat细胞的凋亡情况,Western blot检测FOXO3a磷酸化,免疫荧光检测FOXO3a的核转位核转位。采用si RNA干扰Bim1,观察对Jurkat细胞增殖的影响。结果表明,伏立诺他协同NVP-BEZ235能够促进Jurkat细胞凋亡,降低FOXO3a的磷酸化并诱导其发生核转位,且能诱导Bim1表达,沉默Bim1能逆转伏立诺他和NVP-BEZ235对Jurkat细胞生长的抑制效应。To investigate the effect of vorinostat combined with NVP-BEZ235 on apoptosis of human peripheral blood leukemia T cells(Jurkat cells)and its mechanism,Jurkat cells were cultured and treated with vorinostat or(and)NVP-BEZ235,and then the apoptosis of Jurkat cells was detected by flow cytometry.The phosphorylation of FOXO3a was detected by Western blot,and the nuclear translocation of FOXO3a was detected by immunofluorescence.Bim1 was interfered by si RNA to observe its effect on Jurkat cell proliferation.Vorinostat combined with NVP-BEZ235 can promote Jurkat cell apoptosis.Vorinostat and NVP-BEZ235 can reduce the phosphorylation of FOXO3a and induce its nuclear translocation,and can induce the expression of Bim1.Silencing Bim1 can reverse the inhibitory effects of vorinostat and NVP-BEZ235 on the growth of Jurkat cells.

关 键 词：伏立诺他 NVP-BEZ235 JURKAT细胞 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]