Minor CCI与经典CCI大鼠模型成模特点的比较研究  

Comparison of the characteristics of Minor and classic CCI models

作　　者：王厚融 刘志凤 于天源[1] 张英琦 焦谊 刘迪 徐亚静[1] 官乾 WANG Hourong;LIU Zhifeng;YU Tianyuan;ZHANG Yingqi;JIAO Yi;LIU Di;XU Yajing;GUAN Qian(Beijing University of Chinese Medicine,Beijing 102488;China.2.Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078)

机构地区：[1]北京中医药大学针灸推拿学院,北京102488 [2]北京中医药大学东方医院,北京100078

出　　处：《中国比较医学杂志》2022年第10期1-7,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金项目(82074573,81674094)。

摘　　要：目的比较研究轻度和经典方法诱导的大鼠慢性压迫性神经损伤(chronic constriction injury,CCI)模型的成模特点,为基础研究实验设计提供参考。方法将18只8周龄SD雄性大鼠随机分为假手术组、Minor CCI组和经典CCI组,每组6只。于坐骨神经结扎铬制可吸收外科缝线,Minor CCI组结扎1根,经典CCI组结扎4根。3组动物于造模前和造模后7、14、21、28 d检测机械缩足反射阈值、热缩足反射潜伏期和累积疼痛评分变化情况。透射电镜观察坐骨神经超微结构变化情况。结果Minor CCI组和经典CCI组机械缩足反射阈值和热缩足反射潜伏期痛敏趋势一致,Minor CCI组累积疼痛评分较经典CCI组低。Minor CCI组造模后出现痛敏,造模后14 d进入平台期,约10 d,各症状逐渐恢复;经典CCI组造模后出现痛敏,造模后14 d进入平台期,造模后28 d痛敏依然稳定甚至出现进一步敏化。Minor CCI组和经典CCI组坐骨神经超微结构变化一致,均表现为髓鞘结构破坏、崩脱,形成髓鞘球、自噬体等。结论Minor CCI模型更稳定,痛敏症状可维持至造模后28 d,基本无自残现象,适用于短期实验设计,可最大程度减小实验动物的痛苦。经典CCI模型痛敏症状持久,造模后28 d依然处于平台期,但自残现象明显,适用于中、长期实验设计。Objective To compare the characteristics of the chronic constriction injury model in SD rats induced by Minor and classic CCI,and to provide a reference for the experimental design of basic research.Methods Eighteen 8-week-old SD male rats were randomly divided into sham,Minor CCI,and classic CCI groups with six animals in each group.The 1-ligature was ligated on the sciatic nerve in the Minor CCI group,and the 4-ligature was ligated in the classic CCI group.Animals in the three groups were examined before modeling and at 7,14,21 and 28 days after modeling for the mechanical withdrawal threshold,thermal withdrawal latency,and accumulated pain score.Ultrastructural changes of the sciatic nerve were observed by electron microscopy.Results Nociceptive symptoms of Minor and classic CCI groups were the same in terms of the mechanical withdrawal threshold and thermal withdrawal latency.Additionally,the accumulated pain score of the Minor CCI group was lower than that of the classic CCI group.Ultrastructural changes of the sciatic nerve in Minor and classic CCI groups were the same.Both of them showed destruction and disintegration of the myelin structure,formation of myelin spheres and autophagosomes.Conclusions The Minor CCI model is more stable and nociceptive symptoms can be maintained for up to 28 days after modeling,basically without self-mutilation,which is suitable for short-term experimental design and minimizes the pain of experimental animals.The classic CCI model has long-lasting nociceptive symptoms that remain in the plateau period for 28 days after modeling,but the self-mutilation phenomenon is obvious,which is suitable for medium and long-term experimental designs.

关 键 词：周围神经病理性疼痛 慢性压迫性神经损伤模型 SD大鼠 疼痛 

分 类 号：R-33[医药卫生]