中心静脉导管监测肺动脉压的兔急性肺栓塞模型的制备  

A rabbit model of acute pulmonary embolism using central venous catheter to monitor pulmonary artery pressure

作　　者：龙嘉琪 赵文丽 彭发全 吴蓓 李跃兵 LONG Jiaqi;ZHAO Wenli;PENG Faquan;WU Bei;LI Yuebing(the Second School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou 310053,China;Department of Pathology,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005;Department of Anesthesiology,the Second Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005)

机构地区：[1]浙江中医药大学第二临床医学院,杭州310053 [2]浙江中医药大学附属第二医院病理科,杭州310005 [3]浙江中医药大学附属第二医院麻醉科,杭州310005

出　　处：《中国比较医学杂志》2022年第10期24-31,39,共9页Chinese Journal of Comparative Medicine

基　　金：浙江省中医药科技计划重点项目(2020ZZ012)。

摘　　要：目的通过用单腔中心静脉导管测量实验兔肺动脉压,结合解剖及病理改变,探讨该法制备兔急性肺栓塞模型的可行性和成功率。方法中心静脉导管末端塑形后在导丝引导下经兔颈内静脉置入肺动脉,连接Medlab生物信号采集处理系统行动态肺动脉压监测。经中心静脉导管注射自体血栓,建立兔急性肺栓塞模型。根据目标肺动脉压力来控制注入血栓数量,可得到严重程度和栓塞范围较为一致的急性肺栓塞模型。结果造模成功率100%,栓塞后肺动脉压、血压、心率与栓塞前比较,差异均有统计学意义(P<0.05)。心肺解剖证实导管在肺动脉主干,病理检查发现肺动脉及肺小动脉存在血栓。结论经颈静脉送入中心静脉导管建立兔急性肺栓塞模型,既能保证栓子的完整性,又能够获得连续且准确的肺动脉压力数据,有效地建立了一种栓塞范围和严重程度可控的动物模型。该方法成功率较高、操作简便、使用经济,为急性肺栓塞诊治的相关研究提供了实验基础。Objective To investigate the feasibility and success rate of a rabbit model of acute pulmonary embolism by using a central venous catheter to measure pulmonary artery pressure and assessing the anatomical and pathological changes.Methods The central venous catheter was placed into the rabbit pulmonary artery through the internal jugular vein using a guidewire and was connected to the Medlab biological signal acquisition and processing system for dynamic pulmonary artery pressure monitoring.A model of acute pulmonary embolism with relatively consistent severity and embolization range was created by controlling the number of thrombi injected via the catheter to obtain the target pulmonary artery pressure.Results The modeling success rate was 100%.After embolization,there were significant changes in the pulmonary artery pressure,blood pressure,and heart rate of the rabbits(P<0.05).Cardiopulmonary anatomic examination confirmed that the catheter was in the main pulmonary artery.Pathological examination revealed thrombi in the pulmonary artery and pulmonary arterioles.Conclusions A rabbit model of acute pulmonary embolism was established by inserting a central venous catheter through the jugular vein;this not only ensured the integrity of the thrombi,but also enabled the attainment of continuous and accurate pulmonary artery pressure data and effectively established an animal model with controllable embolization range and severity.This method has a high success rate,is technically simple and economical,and provides an experimental basis for the diagnosis and treatment of acute pulmonary embolism.

关 键 词：急性肺栓塞 肺动脉压 中心静脉导管 动物模型 兔 

分 类 号：R-33[医药卫生]