一测多评法同时测定胆清胶囊中5种成分  

Simultaneous determination of five constituents in Danqing Capsules by QAMS

作　　者：吴春 覃燕 杨慧丽 杨百科 伍庆[3] WU Chun;QIN Yan;YANG Hui-li;YANG Bai-ke;WU Qing(The Second Affiliated Hospital of Guizhou College of Traditional Chinese Medicine,Guiyang 550001,China;Guizhou Shengjitang Pharmaceutical Co.,Ltd.,Guiyang 550001,China;Key Laboratory for Mountain Environment Information System and Environment Protection,Guizhou Normal University,Guiyang 550001,China)

机构地区：[1]贵州中医药大学第二附属医院,贵州贵阳550001 [2]贵州圣济堂制药有限公司,贵州贵阳550001 [3]贵州师范大学山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001

出　　处：《中成药》2022年第11期3445-3449,共5页Chinese Traditional Patent Medicine

基　　金：贵州省教育厅课题—贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2022]264号);贵州省教育厅滚动支持省属高校科研平台团队项目(黔教科[2022]010号);国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401);贵州中药生产过程质量控制研究项目(黔教合KY字[2018]014)。

摘　　要：目的建立一测多评法同时测定胆清胶囊(虎耳草、凤尾草、大黄等)中大黄酸、岩白菜素、大黄酚、没食子酸、大黄素的含量。方法该药物甲醇提取液的分析采用Diamonsil C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相0.1%甲酸-甲醇,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长275 nm。以大黄酸为内标,计算其他4种成分的相对校正因子,测定其含量。结果5种成分在各自范围内线性关系良好(r>0.9990),平均加样回收率98.60%~100.26%,RSD 0.89%~1.26%。一测多评法所得结果与外标法接近。结论该方法简便、准确、可靠,可用于胆清胶囊的质量控制。AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of rhein,bergenin,chrysophanol,gallic acid and emodin in Danqing Capsules(Saxifraga stolonifera Curt.,Pteris multifida Poir,Rhei Radix et Rhizoma,etc.).METHODS The analysis of methanol extract of this drug was performed on a 30℃thermostatic Diamonsil C_(18) column(4.6 mm×250 mm,5μm),with the mobile phase comprising of 0.1%formic acid-methanol flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 275 nm.Rhein was used as an internal standard to calculate the relative correction factors of the other four constituents,after which the content determination was made.RESULTS Five constituents showed good linear relationships within their own ranges(r>0.9990),whose average recoveries were 98.60%-100.26%with the RSDs of 0.89%-1.26%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,accurate and reliable method can be used for the quality control of Danqing Capsules.

关 键 词：胆清胶囊 大黄酸 岩白菜素 大黄酚 没食子酸 大黄素 一测多评 

分 类 号：R927.2[医药卫生—药学]