I TGAV在非小细胞肺癌中的表达及其与放射抵抗的关系  

Expression of ITGAV in Non-small Cell Lung Cancer and Its Relationship with Radioresistance

作　　者：唐园惠 朱圣明[2] 柴晶晶 韩佳慧 田超 邓鑫州 段奇文[2] TANG Yuanhui;ZHU Shengming;CHAI Jingjing;HAN Jiahui;TIAN Chao;DENG Xingzhou;DUAN Qiwen(Hubei University of Medicine,Shiyan 442000,China;Department of Oncology,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China;Postgraduate Training Basement of Jinzhou Medical University Taihe Hospital,Shiyan 442000,China)

机构地区：[1]湖北医药学院,十堰442000 [2]湖北医药学院附属太和医院肿瘤科,十堰442000 [3]锦州医科大学十堰市太和医院研究生培养基地,十堰442000

出　　处：《肿瘤防治研究》2022年第11期1112-1118,共7页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金(81802997);十堰市科技局2021年度引导性科研项目(21Y18);湖北省医药学院研究生科技创新项目(YC2022012)。

摘　　要：目的探讨ITGAV的表达与NSCLC细胞放射敏感度的关系。方法使用生物信息学方法分析ITGAV在NSCLC组织中的表达水平及其与NSCLC患者预后的相关性。克隆形成实验验证放射抵抗细胞与亲本细胞的放射敏感度差异,Western blot检测ITGAV的表达。Western blot、qRT-PCR验证si-ITGAV转染效率,选用最佳ITGAV干扰序列转染A549R、H1299R细胞。克隆形成实验、流式细胞术检测ITGAV干扰后对细胞克隆形成、凋亡和周期情况的影响。结果ITGAV在非小细胞肺癌组织中表达水平明显高于正常组织(P<0.05),且其高表达与患者不良预后有关。ITGAV干扰组细胞在4、6、8 Gy射线照射克隆形成能力与阴性对照组相比显著降低(均P<0.05)。经6 Gy照射后,ITGAV干扰组细胞的凋亡率增加(P_(H1299)R<0.0001,P_(A549)R=0.0002),G_(2)/M期细胞比例明显高于阴性对照组(P_(H1299)R<0.0001,P_(A549)R=0.0007)。结论干扰ITGAV表达可以增加非小细胞肺癌放射敏感度。Objective To investigate the relationship between the expression of ITGAV and the radiosensitivity of NSCLC cells.Methods The expression of ITGAV in NSCLC and its relationship to the prognosis of patients who received radiotherapy were analyzed using bioinformatics methods.Differences in radiosensitivity between radio-resistant cells and parent cells were verified by clone formation experiment,and the protein expression of ITGAV was detected by Western blot.The transfection efficiency of si-ITGAV was determined by Western blot and qRT-PCR analyses.The best ITGAV interference sequence was selected to transfect A549R and H1299R cells.Clone formation experiment and flow cytometry were used to detect clone formation,apoptosis and cell cycle of A549R and H1299R cells.Results The expression of ITGAV in NSCLC tissues was significantly higher than that in normal tissues(P<0.05),and NSCLC patients with high ITGAV expression had poor prognosis.The clonogenic ability of the si-ITGAV group was significantly lower than that of the negative control group at 4,6,8Gy irradiation(all P<0.05).After 6 Gy irradiation,the apoptosis of the si-ITGAV group was increased(P_(H1299)R<0.0001,P_(A549)R=0.0002),the proportion of G_(2)/M phase cells to A549-siITGAV and H1299R-siITGAV cells was higher than that in the negative control group(P_(H1299)R<0.0001,P_(A549)R=0.0007).Conclusion Interfering with ITGAV expression can increase the radiosensitivity of NSCLC.

关 键 词：ITGAV 放射抵抗 肺癌 生物信息学 

分 类 号：R734.2[医药卫生—肿瘤]