circ_0000615靶向调控miR-142-3p对骨肉瘤细胞增殖迁移侵袭的影响  

Effect of circ_0000615 on Proliferation Migration and Invasion of Osteosarcoma Cells by Targeting miR-142-3p

作　　者：李贺伟[1] 阮锋[1] LI Hewei;RUAN Feng(Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei Wuhan 430077,China)

机构地区：[1]华中科技大学同济医学院附属梨园医院,湖北武汉430077

出　　处：《河北医学》2022年第11期1774-1778,共5页Hebei Medicine

基　　金：湖北省武汉市卫生健康委员会资助项目,(编号:武卫通【2019】87号)。

摘　　要：目的:探讨circ_0000615对骨肉瘤细胞增殖、迁移、侵袭的影响及分子机制。方法:选取60例骨肉瘤患者的骨肉瘤组织及瘤旁组织,实时荧光定量PCR(RT-qPCR)检测circ_0000615和miR-142-3p表达水平;将骨肉瘤细胞U2OS分为si-circ_0000615组、si-NC组、miR-142-3p组、miR-NC组、si-circ_0000615+anti-miR-142-3p组、si-circ_0000615+anti-miR-NC组;四甲基偶氮唑盐比色法(MTT)检测细胞抑制率;克隆形成实验检测细胞克隆数;Transwell法检测U2OS细胞迁移和侵袭数;蛋白质印迹法检测蛋白表达;双荧光素酶报告实验检测circ_0000615和miR-142-3p的靶向关系。结果:与瘤旁组织相比,骨肉瘤组织中circ_0000615表达水平升高,miR-142-3p表达水平降低(P<0.05)。沉默circ_0000615或过表达miR-142-3p后,U2OS细胞抑制率、E-cadherin表达水平升高,N-cadherin表达水平降低,U2OS细胞克隆数、迁移侵袭数减少(P<0.05)。circ_0000615靶向调控miR-142-3p;抑制miR-142-3p可减轻circ_0000615对U2OS细胞增殖、迁移和侵袭的抑制作用。结论:沉默circ_0000615可通过靶向调控miR-142-3p抑制骨肉瘤细胞的增殖、迁移和侵袭。Objective:To explore the effect of circ_0000615 on the proliferation,migration and invasion of osteosarcoma cells and its molecular mechanism.Methods:The osteosarcoma tissues and adjacent tissues of 60 patients with osteosarcoma were selected,and real-time fluorescent quantitative PCR(RT-qPCR)was used to detect the expression levels of circ_0000615 and miR-142-3p;the osteosarcoma cells U2OS were divided into si-circ_0000615 group,si-NC group,miR-142-3p group,miR-NC group,si-circ_0000615+anti-miR-142-3p group,si-circ_0000615+anti-miR-NC group;tetramethylazolium salt colorimetric method(MTT)to detect cell inhibition rate;clone formation experiment to detect cell clone number;Transwell method to detect U2OS cell migration and invasion numbers;Western blot method to detect protein expression;dual luciferase reporter experiment to detect the targeting relationship between circ_0000615 and miR-142-3p.Results:Compared with the adjacent tissues,the expression level of circ_0000615 in osteosarcoma tissue was increased,and the expression level of miR-142-3p was decreased(P<0.05).After silencing circ_0000615 or overexpressing miR-142-3p,the inhibition rate of U2OS cells was increased,the number of clones,migration and invasion of U2OS cells were decreased,the expression level of E-cadherin was increased,and the expression level of N-cadherin was decreased(P<0.05).circ_0000615 targets and regulates miR-142-3p;inhibition of miR-142-3p can reduce the inhibitory effect of circ_0000615 on U2OS cell proliferation,migration and invasion.Conclusion:Silencing circ_0000615 can inhibit the proliferation,migration and invasion of osteosarcoma cells through targeted regulation of miR-142-3p.

关 键 词：circ_0000615 miR-142-3p 骨肉瘤 增殖 迁移 侵袭 

分 类 号：R738.1[医药卫生—肿瘤]