ACL的构建及其对结直肠癌HCT116细胞增殖和迁移的作用  

Construction of ALC and its effect on proliferation and migration of colorectal cancer HCT116 cells

作　　者：刘晓敏[1] 朱雯婷 李玉婷[1] 贾梦磊 严鹏科[1] LIU Xiaomin;ZHU Wenting;LI Yuting;JIA Menglei;YAN Pengke(Department of Pharmacy,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou 510145,Guangdong,China)

机构地区：[1]广州医科大学附属第三医院药学部,广东省广州市510145

出　　处：《中南医学科学杂志》2022年第6期818-822,共5页Medical Science Journal of Central South China

基　　金：国家自然科学基金面上项目(81971742);广州市科技计划项目(202102020149);广州市卫生健康科技项目(20201A011096)。

摘　　要：目的制备阿托伐他汀钙脂质体(ACL),探究其对结直肠癌HCT116细胞增殖、迁移的影响。方法采用乳化-溶剂挥发法制备ACL并进行相关参数的测定。以香豆素6为荧光探针探究HCT116细胞摄取的变化;MTT实验和细胞平板克隆实验检测ACL和阿托伐他汀钙(AC)对HCT116细胞增殖的影响;Transwell实验检测ACL和AC对HCT116细胞迁移的影响。结果成功制备ACL,其平均粒径为(87.24±0.485)nm,分散系数为0.240~0.250,Zeta电位为(-12.19±3.642)mV。脂质体形态似球形或圆形结构,外观完整,且大小均匀。脂质体组细胞内荧光强度显著增强,细胞摄取增加。AC和ACL作用后,细胞增殖率和克隆形成率显著降低,迁移细胞数减少,且ACL的抑制作用均显著强于AC。结论ACL可以显著增加HCT116细胞的摄取,增强AC对HCT116细胞的抑制作用。Aim To prepare atorvastatin calcium liposome(ACL)and to investigate its effect on proliferation and migration of colorectal cancer HCT116 cells.Methods The ACL was prepared by emulsification solvent volatilization,and the characteristic of ACL was detected.Coumarin-6 was used as a fluorescent probe to explore the effect on the cellular uptake of HCT116 cells.MTT assay and cell plate cloning assay were used to detect the effects of atorvastatin calcium(AC)and ACL on the proliferation of HCT116 cells.Transwell assay was used to detect the effects of AC and ACL on the migration of HCT116 cells.Results ACL was successfully prepared.The average particle size was(87.24±0.485)nm,dispersion coefficient was 0.240-0.250,the Zeta potential was(-12.19±3.642)mV.The shape of liposome was like spherical or circular structure,with complete appearance and uniform size.The fluorescence intensity of liposome group was significantly enhanced in HCT116 cells,and cellular uptake was increased.After AC and ACL treatment,the cell proliferation rate and clone formation rate decreased significantly,and the number of migrating cells decreased.Moreover,the inhibition of ACL was significantly stronger than that of AC.Conclusion ACL can significantly increase the cell uptake of HCT116 cells and enhance the inhibitory effect of AC on the proliferation and migration of HCT116 cells.

关 键 词：结直肠癌 阿托伐他汀钙脂质体 HCT116细胞 增殖 迁移 

分 类 号：R735.37[医药卫生—肿瘤]