土贝母苷甲通过调控miR-215-5p影响膀胱癌T24细胞增殖、迁移及侵袭  

Effects of tubeimoside-1 on proliferation,migration and invasion of bladder cancer T24 cells by regulating the expression of miR-215-5p

作　　者：杨剑波 曹倪豪 程飞 梁增亮 杨菲 YANG Jianbo;CAO Nihao;CHENG Fei;LIANG Zengliang;YANG Fei(Department of Urology,Nantong Haimen District People's Hospital,Nantong 226100,Jiangsu,China)

机构地区：[1]南通市海门区人民医院泌尿外科,江苏省南通市226100

出　　处：《中南医学科学杂志》2022年第6期837-840,共4页Medical Science Journal of Central South China

基　　金：南通市海门区科技计划项目(2021SF18)。

摘　　要：目的探讨土贝母苷甲通过调控miR-215-5p表达对膀胱癌T24细胞增殖、迁移及侵袭的影响。方法不同质量浓度土贝母苷甲(10、20、30 mg/L)处理人膀胱癌细胞T24。细胞分别转染miR-NC、miR-215-5p mimics、anti-miR-NC、anti-miR-215-5p,随后用土贝母苷甲(30 mg/L)处理T24细胞。CCK-8实验检测细胞增殖;平板克隆形成实验检测细胞克隆形成能力;Transwell实验检测细胞迁移及侵袭;qRT-PCR法检测miR-215-5p的表达;Western blotting法检测基质金属蛋白酶-2(MMP-2)、MMP-9蛋白表达。结果土贝母苷甲作用于T24细胞后,细胞存活率和MMP-2、MMP-9蛋白水平、细胞克隆形成、迁移及侵袭降低(P<0.05),而miR-215-5p表达升高(P<0.05),且呈剂量依赖性递减或递增。miR-215-5p过表达可抑制细胞增殖、克隆形成、迁移及侵袭(P<0.05);抑制miR-215-5p可逆转土贝母苷甲对T24细胞恶性行为的抑制作用。结论土贝母苷甲可通过促进miR-215-5p的表达而抑制膀胱癌细胞恶性行为。Aim To explore the effect of tubeimoside-1 on proliferation,migration and invasion of bladder cancer T24 cells by regulating the expression of miR-215-5p.Methods Different concentrations of tubeimoside-1(10,20,30 mg/L)were used to treat human bladder cancer cells T24.miR-NC,miR-215-5p mimics,anti-miR-NC and anti-miR-215-5p were transfected,and then the T24 cells were treated with tubeimoside-1(30 mg/L).CCK-8 experiment was used to detect cell proliferation.The plate clone formation test was used to detect the ability of cell clone formation.Transwell test detects cell migration and invasion.qRT-PCR method was used to detect the expression of miR-215-5p.Western blotting method was used to detect the protein expression of matrix metalloproteinases-2(MMP-2)and MMP-9.Results After tubeimoside-1 acts on T24 cells,cell viability,protein levels of MMP-2,MMP-9,the formation,migration and invasion of cell clones decreased(P<0.05),the expression of miR-215-5p increased(P<0.05),and decreased or increased in a dose-dependent manner.Transfection of miR-215-5p mimics could inhibit cell proliferation,clone formation,migration and invasion(P<0.05).Transfection of anti-miR-215-5p could reduce the inhibitory effects of tubeimoside-1 on the malignant behavior of T24 cells.Conclusion Tubeimoside-1 could inhibit the malignant behavior of bladder cancer cells by promoting miR-215-5p expression.

关 键 词：膀胱癌 土贝母苷甲 miR-215-5p 细胞增殖 迁移 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]