circUTRN24通过肝星状细胞自噬介导胆道闭锁肝纤维化  

circUTRN24 mediates liver fibrosis in biliary atresia through hepatic stellate cell autophagy

作　　者：刘冬[1] 高家辉 张曦耘 梅倩倩 吴宙光[1] LIU Dong;GAO Jia-hui;ZHANG Xi-yun;MEI Qian-qian;WU Zhou-guang(Department of General Surgery,Shenzhen Children's Hospital,Shenzhen 518026,China)

机构地区：[1]深圳市儿童医院普外科,广东深圳518026

出　　处：《岭南现代临床外科》2022年第5期446-450,共5页Lingnan Modern Clinics in Surgery

基　　金：广东省医学科学技术研究基金项目(A2021364);深圳市儿童医院基金项目(ynkt2020-zz12)。

摘　　要：目的探究胆道闭锁中circUTRN24与自噬途径的关联,以及是否通过自噬参与胆道闭锁的肝纤维化过程。方法通过RT-qPCR、Western blot分别检测BA组和对照组的肝组织中circUTRN24以及自噬相关蛋白的表达,并进行相关性分析。在细胞水平,通过慢病毒转染使人肝星状细胞系LX-2过表达circUTRN24,Western blot检测转染前后自噬相关蛋白Beclin-1、LC3以及HSC活化相关指标α-SMA、COL-Ⅰ的表达水平。结果胆道闭锁患儿肝组织中circUTRN24以及自噬相关蛋白Beclin-1的表达明显高于对照组,并且呈正相关关系。过表达circUTRN24的LX-2细胞中自噬相关蛋白Beclin-1、LC3-Ⅱ及HSC活化相关指标α-SMA、COL-Ⅰ均明显升高。结论circUTRN24可能通过调控肝星状细胞自噬过程促进胆道闭锁肝纤维化的进展。Objective To explore the relationship between circUTRN24 and autophagy in biliary atresia,and whether circUTRN24 participates in the process of liver fibrosis in biliary atresia through autophagy.Methods The expressions of circUTRN24 and autophagy related proteins in liver tissues of BA group and CC group were detected by RT-qPCR and Western blot,and the correlation was analyzed.At the cellular level,human hepatic stellate cell line LX-2 overexpressed circUTRN24 by lentivirus transfection.Western blot was used to detect the expression of autophagy related proteins Beclin-1,LC3and HSC activation related factorsα-SMA and COL-Ⅰ before and after transfection.Results The expressions of circUTRN24 and autophagy related proteins Beclin-1 in the liver tissue of children with biliary atresia were significantly higher than those in the control group,and there was a positive correlation between them.Overexpression of circUTRN24 in LX-2 cells significantly increased autophagy related proteins(Beclin-1 and LC3-Ⅱ)and HSC activation related factors(α-SMA and COL-Ⅰ).Conclusion circUTRN24 may promote the progression of hepatic fibrosis in biliary atresia by regulating the autophagy of hepatic stellate cells.

关 键 词：环状RNA 肝星状细胞 自噬 胆道闭锁 肝纤维化 

分 类 号：R735.7[医药卫生—肿瘤]