浙江省岱山县一起肾综合征出血热疫情汉坦病毒分子流行病学分析  

Molecular epidemiological analysis of Hantavirus in an outbreak of hemorrhagic fever with renal syndrome in Daishan county,Zhejiang province,China

作　　者：陈旭辉 谭启龙[2] 舒纪为 叶凌[2] 李世波 王秋景 CHEN Xu-hui;TAN Qi-long;SHU Ji-wei;YE Ling;LI Shi-bo;WANG Qiu-jing(Laboratory of Daishan First People’s Hospital,Zhoushan,Zhejiang 316200,China;Department of Infectious Disease Control and Prevention,Daishan Center for Disease Control and Prevention,Zhoushan,Zhejiang 316200,China;Department of Infectious Disease,Zhoushan Hospital,Wenzhou Medical University,Zhoushan,Zhejiang 316000,China)

机构地区：[1]岱山县第一人民医院检验科,浙江舟山316200 [2]岱山县疾病预防控制中心传染病预防与控制科,浙江舟山316200 [3]温州医科大学附属舟山医院感染科,浙江舟山316000

出　　处：《中国媒介生物学及控制杂志》2022年第5期706-709,共4页Chinese Journal of Vector Biology and Control

基　　金：浙江省自然科学基金(LY21H100002);舟山市科技计划项目(2021C31089)。

摘　　要：目的检测分析2020年浙江省岱山县某海岛工业园肾综合征出血热(HFRS)聚集性疫情病例及宿主动物标本,以明确病例和宿主间的关联及病毒基因进化特点,为当地该病的防治提供参考依据。方法采集病例与鼠血清,采用反转录聚合酶链式反应(RT-PCR)检测病毒核酸,巢式PCR扩增M基因并进行序列测定、基因分型和同源性分析。结果2020年4-7月岱山县某海岛工业园区共报告4例HFRS确诊病例,抗汉坦病毒IgG和IgM均阳性。2例病例和4只褐家鼠血清标本检测到汉坦病毒RNA,均为首尔型病毒(SEOV)。6个M基因部分片段100%同源,与河北省分离株Rod/2012/QHD/4/Gc、浙江省瑞安分离株RuianRn180的亲缘关系最近。结论此聚集性疫情经褐家鼠传播的汉坦病毒SEOV引起,病毒表现高度同源性和明显的地理聚集现象及对宿主的相对选择性。Objective To analyze cases’and host animals’specimens in a cluster outbreak of hemorrhagic fever with renal syndrome(HFRS)in an island industrial park in Daishan county,Zhejiang province,China in 2020,clarify the relationship between the cases and hosts and the molecular phylogenetic characteristics of the virus,and to provide a reference for local prevention and treatment of HFRS.Methods Serum samples from the cases and rodents were collected.Reverse transcription PCR was used to detect the viral nucleic acid.Nested PCR was used to amplify the M gene,followed by sequencing,genotyping,and homology analysis.Results From April to July 2020,a total of four confirmed cases of HFRS were reported in the island industrial park in Daishan county,and all were positive for antihantavirus IgG and IgM antibodies.Hantavirus RNA was present in the serum samples of two cases and four Rattus norvegicus rodents,all being Seoul virus(SEOV).The six samples’M gene fragments were 100%homologous,and were most closely related to the isolates Rod/2012/QHD/4/Gc from Hebei province and RuianRn180 from Ruian,Zhejiang.Conclusion This cluster outbreak was caused by the transmission of SEOV hantavirus through R.norvegicus.The virus strains exhibited high homology,obvious geographic clustering,and relative selectivity for hosts.

关 键 词：汉坦病毒 检测 基因测序 进化分析 

分 类 号：R373.32[医药卫生—病原生物学]