巴西苏木素下调膀胱癌T24细胞和BIU87细胞细胞质分裂调控蛋白1的表达及抑制细胞增殖的研究  

Brazilin downregulates the expression of protein regulator of cytokinesis 1 in bladder cancer T24 cell and BIU87 cell and inhibits the cell proliferation

作　　者：杨喜花[1] 赵莉莉[1] 陈丽霞 杨永明[1] 王靖[1] 阎磊[1] 韩雪冰[2] Yang Xihua;Zhao Lili;Chen Lixia;Yang Yongming;Wang Jing;Yan Lei;Han Xuebing(Laboratory Animal Center,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China;Department of Urology,Shanxi Province Cancer Hospital,Shanxi Hospital Affiliated to Cancer Hospital,Chinese Academy of Medical Sciences,Cancer Hospital Affiliated to Shanxi Medical University,Taiyuan 030013,China)

机构地区：[1]山西省肿瘤医院,中国医学科学院肿瘤医院山西医院,山西医科大学附属肿瘤医院实验动物中心,太原030013 [2]山西省肿瘤医院,中国医学科学院肿瘤医院山西医院,山西医科大学附属肿瘤医院泌尿外科,太原030013

出　　处：《肿瘤研究与临床》2022年第9期661-664,共4页Cancer Research and Clinic

基　　金：国家自然科学基金(82003810);山西省重点研发计划项目(201803D31177);山西省卫生健康委员会"四个一批"科技兴医创新计划(2020SYS25)。

摘　　要：目的探讨巴西苏木素对膀胱癌细胞的抑制作用及其机制。方法采用化学合成法制备巴西苏木素。采用四甲基偶氮唑盐(MTT)法检测合成的巴西苏木素对膀胱癌T24细胞和BIU87细胞增殖的抑制作用, 采用蛋白质组学技术检测巴西苏木素对两种细胞蛋白表达水平的影响, 采用实时荧光定量聚合酶链反应(qRT-PCR)及蛋白质印迹法验证巴西苏木素对两种细胞的细胞质分裂调控蛋白1(PRC1)基因水平和蛋白水平表达的影响。结果 MTT法检测显示, 巴西苏木素可明显抑制膀胱癌T24和BIU87细胞的增殖, 其对T24细胞和BIU87细胞的半抑制浓度(IC50)分别为9.9 μg/ml和5.1 μg/ml;蛋白质组学检测结果显示, 巴西苏木素可下调两种细胞中PRC1蛋白的表达, 并经qRT-PCR及蛋白质印迹法得到了验证。结论巴西苏木素可能通过下调PRC1表达抑制膀胱癌细胞的增殖。Objective To investigate the inhibitory effect of brazilin on bladder cancer cells and its mechanism.Methods Chemically synthesized brazilin was synthesized by chemical synthesis.Methyl thiazolyl tetrazolium(MTT)method was used to detect the inhibitory effect of synthetic brazilin on bladder cancer cells T24 and BIU87.Proteomic technique was used to detect the effect of brazilin on the level of protein in both cells.Real-time quantitative polymerase chain reaction(qRT-PCR)and Western blot methods were used to verify the effects of brazilin on the expression of protein regulator of cytokinesis 1(PRC1)of both cells at gene and protein level.Results MTT method showed that brazilin significantly inhibited the proliferation of bladder cancer cells T24 and BIU87,and its half inhibitory concentration(IC50)of T24 cell and BIU87 cell was 9.9µg/ml and 5.1µg/ml,respectively.Proteomic results showed that brazilin could regulate the protein expression of PRC1 in both cells,which was verified by qRT-PCR and Western blot.Conclusion Brazilin suppresses bladder cancer cell growth possibly by downregulating PRC1.

关 键 词：膀胱肿瘤 巴西苏木素 细胞质分裂调控蛋白1 蛋白质组学 细胞增殖 

分 类 号：R737.14[医药卫生—肿瘤]