急性脑梗死患者血清Beclin-1,3-MST,TSP-1表达水平及与病情和预后的相关性研究  

Serum Beclin-1,3-MST and TSP-1 Expression Levels in Patients with Acute Cerebral Infarction and Their Correlation with Disease Condition and Prognosis

作　　者：张磊 赵腾跃 李彬[2] ZHANG Lei;ZHAO Teng-yue;LI Bin(Department of Neurology,the Second Hospital of Zhangjiakou City,Hebei Zhangjiakou 075000,China;Department of Neurology,Baoding Cerebrovascular Disease Hospital,Hebei Baoding 071000,China)

机构地区：[1]张家口市第二医院神经内科,河北张家口075000 [2]保定市脑血管病医院神经内科,河北保定071000

出　　处：《现代检验医学杂志》2022年第6期124-128,148,共6页Journal of Modern Laboratory Medicine

基　　金：保定市科技计划项目(2041ZF281)。

摘　　要：目的探讨急性脑梗死(acute cerebral infarction,ACI)患者血清自噬相关蛋白Beclin-1,巯基丙酮酸硫基转移酶(3-mercaptopyruvate sulfurtransferase,3-MST)和凝血酶敏感蛋白-1(thrombospondin-1,TSP-1)表达水平及与病情和预后的相关性研究。方法选取2019年6月~2021年6月于张家口市第二医院神经内科就诊的ACI患者150例作为研究组,按脑梗死面积大小分为小面积组(71例)、中面积组(42例)和大面积组(37例),按神经损伤程度分为轻度损伤组(75例)、中度损伤组(38例)和重度损伤组(37例),按二个月内生存结局分为存活组(113例)和死亡组(37例)。另选取100例同期体检健康者作为对照组。检测Beclin-1,3-MST和TSP-1在各组研究对象血清中表达水平的变化,分析三者与ACI患者预后的关系。结果研究组患者血清Beclin-1(2.13±0.57 ng/ml),TSP-1(171.45±31.66 ng/ml)水平明显高于对照组(0.88±0.29 ng/ml,86.29±20.52 ng/ml),而3-MST(3.05±0.51 ng/ml)水平明显低于对照组(5.11±0.79 ng/ml),差异均有统计学意义(t=20.234,23.767,25.061,均P<0.001)。血清Beclin-1,TSP-1表达水平大面积组(3.14±0.67 ng/ml,231.91±39.43 ng/ml)高于中面积组(2.15±0.59 ng/ml,201.49±33.19 ng/ml)和小面积组(1.59±0.33 ng/ml,122.17±25.79 ng/ml),中面积组高于小面积组,差异均有统计学意义(F=112.922,171.981,均P<0.001);重度损伤组(3.55±0.51 ng/ml,256.39±37.99 ng/ml)高于中度损伤组(2.29±0.69 ng/ml,203.61±35.97 ng/ml)和轻度损伤组(1.35±0.48 ng/ml,113.25±30.19 ng/ml),中度损伤组高于轻度损伤组,差异均有统计学意义(F=202.342,246.191,均P<0.001);死亡组Beclin-1,TSP-1表达水平(3.15±0.78 ng/ml,277.49±39.55 ng/ml)高于存活组(1.79±0.68 ng/ml,136.73±30.78 ng/ml),差异均有统计学意义(t=10.176,22.433,均P<0.001)。血清3-MST表达水平,大面积组(0.96±0.26 ng/ml)低于中面积组(3.84±0.89 ng/ml)和小面积组(4.67±0.97 ng/ml),中面积组低于小面积组,差异有统计学意义(F=248.483,均P<0.001);重Objective To investigate the serum autophagy-related protein Beclin-1,3-mercaptopyruvate sulfurtransferase(3-MST)and thrombin sensitive protein-1(TSP-1)expression levels in patients with acute cerebral infarction(ACI)and their correlation with the disease and prognosis.Methods A total of 150 ACI patients admitted to the Department of Neurology of the Second Hospital of Zhangjiakou from June 2019 to June 2021 were selected as the study group.According to the size of cerebral infarction,they were divided into small area group(71 cases),medium area group(42 cases)and large area group(37 cases),and according to the degree of nerve injury,they were divided into mild injury group(75 cases),moderate injury group(38 cases),severe injury group(37 cases),survival group(113 cases)and death group(37 cases)according to the survival outcome within 2 months.Another 100 healthy subjects were selected as the control group.The expression levels of Beclin-1,3-MST and TSP-1 in each group were detected,and the relationship between Beclin-1,3-MST and TSP-1 and prognosis of ACI patients was analyzed.Results The serum levels of Beclin-1(2.13±0.57 ng/ml)and TSP-1(171.45±31.66 ng/ml)in the study group were significantly higher than those in the control group(0.88±0.29 ng/ml,86.29±20.52 ng/ml),the level of 3-MST(3.05±0.51 ng/ml)was significantly lower than that of the control group(5.11±0.79 ng/ml),the differences were statistically significant(t=20.234,23.767,25.061,all P<0.001).The expression levels of Beclin-1 and TSP-1 in large area group(3.14±0.67 ng/ml,231.91±39.43 ng/ml)were higher than those in medium area group(2.15±0.59 ng/ml,201.49±33.19 ng/ml)and small area group(1.59±0.33 ng/ml,122.17±25.79 ng/ml).Medium area group was higher than small area group,the differences were statistically significant(F=112.922,171.981,all P<0.001).The severe injury group(3.55±0.51 ng/ml,256.39±37.99 ng/ml)was higher than the moderate injury group(2.29±0.69 ng/ml,203.61±35.97 ng/ml).The mild injury group(1.35±0.48 ng/ml,113.25±30.19

关 键 词：急性脑梗死 自噬相关蛋白Beclin-1 巯基丙酮酸硫基转移酶 凝血酶敏感蛋白-1 

分 类 号：R743.33[医药卫生—神经病学与精神病学] R392.11[医药卫生—临床医学]