TBX2在卵巢癌中的表达及对卵巢癌细胞生物学特性影响的研究  

The expression of TBX2 in ovarian cancer and its effect on the biological characteristics of ovarian cancer cell

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作  者:任亚男 王雅卓 张俊勤[1] 范佳佳 李新君[1] 褚兆苹[1] REN Yanan;WANG Yazhuo;ZHANG Junqin;FAN Jiajia;LI Xinjun;CHU Zhaoping(Department of Gynecology,Hebei Provincial People's Hospital,Shijiazhuang,Hebei 050000,China)

机构地区:[1]河北省人民医院妇科,河北石家庄050000

出  处:《中国优生与遗传杂志》2022年第9期1513-1518,共6页Chinese Journal of Birth Health & Heredity

基  金:河北省医学科学研究课题(20190207)。

摘  要:目的探讨T-box转录因子2(TBX2)在卵巢癌中的表达及对卵巢癌细胞生长、细胞周期和凋亡的影响。方法免疫组化检测卵巢癌组织和正常卵巢组织中TBX2的表达。细胞实验分为正常组、转染TBX2-siRNA-NC为对照组细胞、转染TBX2-siRNA为实验组细胞。电镜观察各组细胞的超微结构,平板克隆实验检测各组细胞的生长活性,流式细胞术检测细胞周期的变化,Hoechst 33258染色检测各组细胞的凋亡率。结果与正常卵巢组织相比,卵巢癌组织中TBX2的表达明显升高(P<0.05);与正常组相比,沉默TBX2的表达后,实验组SKOV3细胞的克隆形成率明显降低,处于G1期的SKOV3细胞的比例明显升高,处于S期细胞的比例明显下降,凋亡率明显升高,差异均具有统计意义(均P<0.05)。电镜观察结果显示,实验组SKOV3细胞的超微结构损伤严重,胞浆中完整细胞器的数量明显下降,线粒体嵴结构缺失明显,核膜溶解或缺损严重,核仁固缩严重。结论TBX2在卵巢癌中存在高表达的现象,抑制TBX2的表达,破坏肿瘤细胞的超微结构,能明显抑制癌细胞的生长,阻滞其细胞周期,诱导细胞的凋亡。Objective To investigate the expression of T-box transcription factors 2(TBX2)in ovarian cancer and its effect on the growth,cell cycle and apoptosis of ovarian cancer cells.Methods Immunohistochemistry was used to detect the expression of TBX2 in ovarian cancer tissues and normal ovarian tissues.The cells experiments were divided into normal group,cells transfected with TBX2-siRNA-NC were control group and cells transfected with TBX2-siRNA were experimental group.The electron microscope was used to detect the ultrastructure of each group of cells.Plate cloning experiment was used to detect the growth activity of each group of cells.Flow cytometry was used to detect the cell cycle of each group of cells.Hoechst 33258 staining was used to detect the apoptosis rate of each group of cells.Results Compared with normal ovarian tissues,the expression of TBX2 in ovarian cancer tissues was significantly increased(P<0.05).Compared with the normal group,after silencing the expression of TBX2,the cloning rate of SKOV3 cells in the experimental group was significantly reduced.The proportion of SKOV3 cells,in the G1 stage,was significantly increased,the proportion of cells in S phase was significantly decreased,and the apoptotic rate was significantly increased.The differences were statistically significant(all P<0.05).Electron microscope observations showed that the ultrastructure damage of the cells in the experimental group was obvious,the number of cytoplasmic organelles decreased,the vacuolar degeneration of mitochondria was obvious,the cristae structure was obviously missing,the nuclear membrane was seriously damaged or damaged,and the nucleolus was severely contracted.Conclusion TBX2 is highly expressed in ovarian cancer.Inhibiting the expression of TBX2,and destroying the ultrastructure of tumor cells can significantly inhibit the growth of cancer cells,block the cell cycle and induce cell apoptosis.

关 键 词:T-box转录因子2 卵巢癌 细胞生长 细胞周期 凋亡 

分 类 号:R737.31[医药卫生—肿瘤]

 

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