霉菌毒素吸附剂与有机酸或植物精油的复合物对蛋鸡肠道黏膜屏障功能的影响  

Effects of Mycotoxin Adsorbent-Organic Acid Complex and Mycotoxin Adsorbent-Essential Oil Complex on Intestinal Mucosal Barrier Function of Laying Hens

作　　者：陈继发 张佳鑫 CHEN Jifa;ZHANG Jiaxin(Selenium Agricultural Engineering Technology Research Center of Jiangxi Provincial Colleges and Universities,College of Life Science and Resources and Environment,Yichun University,Yichun 336000,China)

机构地区：[1]宜春学院生命科学与资源环境学院,江西省高等学校硒农业工程技术研究中心,宜春336000

出　　处：《动物营养学报》2022年第11期6994-7001,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：江西省教育厅科技项目(GJJ211624);宜春学院引进高层次人才科研启动基金(3360119022)。

摘　　要：本试验旨在研究霉菌毒素吸附剂(MA)与有机酸(OA)或植物精油(EO)的复合物对蛋鸡肠道黏膜屏障功能的影响。选取270只健康的29周龄罗曼蛋鸡,随机分为3组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.65 g/kg MA-OA复合物、0.70 g/kg MA-EO复合物的试验饲粮。预试期1周,正试期10周。结果表明:与对照组相比,饲粮添加MA-OA复合物或MA-EO复合物显著降低了回肠产气荚膜梭菌相对数量(P<0.05),显著下调了回肠黏膜髓样分化因子88和白细胞介素-1βmRNA相对表达水平(P<0.05),显著提高了回肠蔗糖酶活性和绒毛高度/隐窝深度值(P<0.05);饲粮添加MA-EO复合物显著上调了回肠黏膜闭合蛋白-5(claudin-5) mRNA相对表达水平(P<0.05);饲粮添加MA-OA复合物显著降低了回肠沙门氏菌相对数量(P<0.05),显著下调了回肠黏膜核转录因子-κB p65 mRNA相对表达水平(P<0.05),显著提高了回肠碱性磷酸酶活性和空肠绒毛高度/隐窝深度值(P<0.05)。由此可见,饲粮添加MA-OA复合物或MA-EO复合物均可提高蛋鸡的肠道屏障功能,改善肠道健康。This study was conducted to investigate the effects of mycotoxin adsorbent(MA)-organic acid(OA)complex and MA-essential oil(EO)complex on intestinal mucosal barrier function of laying hens.A total of 270 healthy 29-week-old laying hens were randomly divided into 3 groups with 6 replicates per group and 15 hens per replicate.Hens in the control group were fed a basal diet,and hens in the test groups were fed the basal diet supplemented with 0.65 g/kg MA-OA complex and 0.70 g/kg MA-EO complex,respectively.The adjustment period lasted for 1 week,and the experimental period lasted for 10 weeks.The results showed as follows:compared with the control group,dietary supplemented with both MA-OA complex or MA-EO complex significantly reduced the relative amount of Clostridium perfringens in ileum(P<0.05),and significantly down-regulated the mRNA relative expression levels of myeloid differentiation factor 88(MyD88)and interleukin-1β(IL-1β),while significantly increased the sucrase activity and villus height/crypt depth ratio in ileum(P<0.05).Besides,dietary supplemented with MA-EO complex significantly up-regulated the mRNA relative expression level of claudin-5 in ileal mucosa(P<0.05);dietary supplemented with MA-OA complex significantly reduced the relative amount of Salmonella in ileum(P<0.05),and significantly down-regulated the mRNA relative expression level of nuclear factor kappa B p65(NF-κB p65)in ileal mucosa(P<0.05),while significantly enhanced the alkaline phosphatase activity in ileum and villus height/crypt depth ratio in jejunum(P<0.05).In conclusion,dietary supplemented with MA-OA complex or MA-EO complex can improve intestinal mucosal barrier function,which contributes to the health of the intestine in laying hens.[Chinese Journal of Animal Nutrition,2022,34(11):6994-7001]

关 键 词：霉菌毒素吸附剂 有机酸 植物精油 蛋鸡 肠道屏障 

分 类 号：S831[农业科学—畜牧学]