紫象草原花青素提取物的制备条件优化和体外抗氧化活性研究  

Optimization of Preparation Conditions and in Vitro Antioxidant Activity of Proanthocyanidin Extract from Pennisetum purpureum Schumab

作　　者：刘赛格 冉思蛟 倪海花 杜勇 高彦华 陈仕勇[1] LIU Saige;RAN Sijiao;NI Haihua;DU Yong;GAO Yanhua;CHEN Shiyong(Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resources Reservation and Utilization,Key Laboratory of Animal Science of National Ethnic Affairs Commission of China,College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China)

机构地区：[1]西南民族大学畜牧兽医学院动物科学国家民委重点实验室青藏高原动物遗传资源保护与利用教育部重点实验室,成都610041

出　　处：《动物营养学报》2022年第11期7443-7454,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：四川省2021—2023年高等教育人才培养质量和教学改革项目(JG2021-414);国家级大学生创新创业训练计划支持项目(202110656018);西南民族大学中央高校基本科研业务专项(2021PTJS26)。

摘　　要：本试验旨在探究紫象草原花青素提取物的最佳制备条件,建立快速灵敏测定提取物含量的方法,并研究其体外抗氧化活性。首先建立对-(二甲基氨基)肉桂醛(DMAC)测定紫象草原花青素提取物含量的方法,在此基础上进行提取条件的单因素筛选试验,并对料液比、提取温度、提取时间3个因素进行响应面优化,探究最优参数。按照最优参数制备紫象草原花青素提取物并测定其对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS·)、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、羟自由基(OH·)的体外清除率。结果表明:紫象草原花青素提取物的最优制备条件参数为料液比1∶110 (g/mL)、提取温度55℃、提取时间60 min,提取得到原花青素B2含量为5.06 mg/g。紫象草原花青素提取物浓度为200 mg/mL时对ABTS·、DPPH·清除率分别为95.69%、96.79%,浓度为25 mg/mL时,对OH·清除率为90.04%。综上所述,本试验建立了一种实验室快速、灵敏测定紫象草原花青素提取物含量的方法,通过响应面法优化提取条件制备的原花青素提取物具有较好的体外抗氧化活性。This experiment was conducted to explore the optimal preparation conditions of proanthocyanidin extract from Pennisetum purpureum Schumab,to establish a rapid and sensitive method to determine the extract content,and to evaluate the in vitro antioxidant activity.Firstly,a p-(dimethylamino)cinnamaldehyde(DMAC)method was established to determine the concentration of proanthocyanidin extract from Pennisetum purpureum Schumab.On this basis,a single factor screening test was carried out to determine the extraction conditions,and response surface analysis was utilized to optimize the parameters of solid-liquid ratio,extraction temperature and extraction time.Finally,the proanthocyanidin extract from Pennisetum purpureum Schumab samples were prepared according to the optimal conditions and their in vitro scavenging rates of 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt radical(ABTS·),1,1-diphenyl-2-picrylhydrazyl radical(DPPH·)and hydroxy radical(OH·)were analyzed.The result showed that the optimal preparation condition parameters of proanthocyanidin extract from Pennisetum purpureum Schumab were as follows:solid-liquid ratio,1∶110 g/mL;extraction temperature 55 ℃ and extraction time 60 min,and the extracted proanthocyanidin B2 content was 5.06 mg/g.When the proanthocyanidin extract from Pennisetum purpureum Schumab concentration was 200 mg/mL,the scavenging rates of ABTS·and DPPH·were 95.69%and 96.79%,respectively;when the concentration was 25 mg/mL,the scavenging rates of OH·was90.04%.In summary,this study establishes a rapid and sensitive laboratory method for the concentration determination of proanthocyanidin extract from Pennisetum purpureum Schumab,and the proanthocyanidin extract from Pennisetum purpureum Schumab prepared using optimize the extraction conditions by response surface analysis show relatively good antioxidant activities in vitro.[Chinese Journal of Animal Nutrition,2022,34(11):7443-7454]

关 键 词：紫象草 原花青素 提取条件优化 响应面分析 体外抗氧化活性 

分 类 号：S816[农业科学—饲料科学]