分光光度法与液相色谱-质谱联用法测定特殊医学用途婴儿配方奶粉中左旋肉碱比较  

Comparison of Spectrophotometry and LC-MS/MS in Determination of L-Carnitine in Infant Formula for Special Medical Use

作　　者：陈伟杰 叶妍 CHEN Weijie;YE Yan(South China Agricultural University,Guangzhou 510642,China;Shenzhen Academy of Metrology&Quality Inspection,Shenzhen 518131,China)

机构地区：[1]华南农业大学,广东广州510642 [2]深圳计量质量检测研究院,广东深圳518131

出　　处：《食品安全导刊》2022年第32期60-63,共4页China Food Safety Magazine

摘　　要：本文以常见类型的特殊医学用途配方奶粉为研究对象,对《食品安全国家标准婴幼儿食品和乳品中左旋肉碱的测定》(GB 29989-2013)、淀粉酶酶解优化分光光度法及液相色谱串联质谱法测定左旋肉碱的结果进行比较分析。结果表明,GB 29989-2013测定这类样品中左旋肉碱过滤困难、数据偏差大且测定结果偏低,淀粉酶酶解优化分光光度法及液相色谱串联质谱法测定结果无显著性差异,快速、准确,适用于特殊医学用途配方食品中左旋肉碱的测定。In this paper,the common types of formula milk powder for special medical purposes were taken as the research object,and the results of L-carnitine of GB 29989-2013,amylase enzymatic optimization spectrophotometry and LC-MS/MS determination were compared.The results showed that the determination of L-carnitine in such samples by GB 29989-2013 was difficult to filter,the RSD was large and the determined results was low.There was no significant difference between the results of optimization spectrophotometry and LC-MS/MS determination.The method was rapid and accurate,and was suitable for the determination of L-carnitine in formula foods for special medical purposes.

关 键 词：左旋肉碱 分光光度法 液相色谱串联质谱法 特殊医学用途配方奶粉 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程] O657[轻工技术与工程—食品科学与工程]