菱角壳木质素提取分离及其抗氧化活性评价  

Evaluation of Antioxidant Activity of Lignin Extracted from Water Caltrop Shell

作　　者：李锋 毛海立[1,2] 曾承露 徐平[1] LI Feng;MAO Hai-li;ZENG cheng-lu;XU ping(School of Chemistry and Chemical Engineering,Qiannan Normal College for Nationalities,Duyun 558000,China;Qiannan Engingineering Research Center for Environmental Governanen and Contamination Control,Qiannan Normal College for Nationalities,Duyun 558000,China)

机构地区：[1]黔南民族师范学院化学化工学院,贵州都匀558000 [2]黔南民族师范学院黔南环境治理与污染控制工程研究中心,贵州都匀558000

出　　处：《化学试剂》2022年第12期1768-1774,共7页Chemical Reagents

基　　金：贵州省教育厅青年科技人才成长资助项目(黔教合KY[2020]204);贵州省科技计划资助项目(黔科合基础-ZK[2022]一般545);黔南民族师范学院引进高层次人才研究资助专项项目(QNSY2019RC11);贵州省普通高等高校工程研究中心资助项目(黔教合KY字[2018]487号)。

摘　　要：为研究菱角壳木质素的结构和功能特性,采用4种方法提取分离菱角壳中木质素,利用紫外吸收光谱、凝胶渗透色谱、傅里叶变换红外光谱和热重分析进行木质素结构表征,并对其抗氧化活性进行评价。结果表明:4种方法提取的菱角壳木质素结构保留完整,主要有紫丁香基和愈创木酚基结构单元组成。除碱木质素外,其他木质素的相对分子量较低,且分散度均小于2.5。热重分析表明菱角壳木质素热解过程主要发生在200~500℃之间。此外,4种木质素均表现出良好的抗氧化活性,其中在木质素浓度为0.4 mg/mL时,AAL、p-TsOH和Bet/LA对DPPH自由基清除率分别可达84.63%、86.62%和62.3%。通过研究以期为菱角壳组分分离和高值化开发利用提供技术支撑。Four lignin samples were isolated and their structural characteristic were investigated using ultraviolet spectroscopy(UV),gel permeation chromatography(GPC),infrared spectroscopy(FT-IR)and thermogravimetric analysis(TGA).The evaluation of the antioxidant activity showed that the benzene ring of lignin with syringl and guaiacyl units remained complete.Compared to AL,the other lignin samples presented low relative molecular mass and polydispersity index(PDI<2.5).TGA showed that the thermal stability of lignin samples was excellent,and most of them degraded in the temperature range of 200~500℃.Moreover,these four samples exhibited distinct antioxidant activity.Under the lignin concentration of 0.4 mg/mL,the capability of AAL,p-TsOH,and Bet/LA samples to scavenge DPPH radical were up to 84.63%,86.62% and 62.3%,respectively.These findings provide a novel way to fractionate lignin from water caltrop shell,contributing to extend the opportunities of lignin valorization into added-value products.

关 键 词：菱角壳 木质素 结构表征 抗氧化活性 热解 

分 类 号：TQ35[化学工程]