Primary culture and identification about brain microvascular endothelial cells of rabbits  

作　　者：MA Hua-gen LIU Zhao-de LIU Hai-qin TANG Yuan-yu 

机构地区：[1]College of TCM,Beijing University of Chinese Medicine,Beijing 102488,China [2]Basic Medical College,Nanjing Medical University,Nanjing 211166,China [3]College of Integrated Traditional Chinese and Western Medicine,Fujian University of TCM,Fuzhou 350122,China [4]College of TCM,Fujian University of TCM,Fuzhou 350122,China

出　　处：《Journal of Hainan Medical University》2022年第19期6-10,共5页海南医学院学报（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(81072714);the Natural Science Foundation of Fujian Province,China(No.2017J01545)。

摘　　要：Objective:To establish a simple and efficient culture method of primary rabbit brain microvascular endothelial cells,provide important carriers and tool cells for the research of related cerebrovascular diseases.Methods:The cerebral cortexes of rabbits were collected aseptic and inoculated after cutting,passing through cell sieve,bovine serum albumin density gradient centrifugation,typeⅡcollagenase digestion,finally inoculated and cultured.The cultured cells were identified by cell morphological observation and angiogenesis experiment.Results:Under the inverted microscope,the cells were short fusiform or polygonal,and grew in clusters and adhere to the wall.After the cells were densely fused,they would be in a typical monolayer flat,“pebbled"mosaic arrangement.Tube formation test had the ability to form tubes structure.Conclusion:This method can successfully separate and cultivate primary rabbit brain microvascular endothelial cells.

关 键 词：RABBIT BRAIN Microvascular endothelial cells Primary culture Morphologic observation Tube formation test 

分 类 号：R-33[医药卫生]