Protective effect and mechanism of clemastine fumarate on acute lung injury in mice with intestinal ischemia-reperfusion  

作　　者：LIU Yang LIU Jie-ting WANG Ying-bin 

机构地区：[1]Department of Anesthesiology,Lanzhou University Second Hospital,Lanzhou 730030,China [2]The Second Clinical Medical School,Lanzhou University,Lanzhou 730030,China

出　　处：《Journal of Hainan Medical University》2022年第19期18-22,共5页海南医学院学报（英文版）

基　　金：This study was supported by Natural Science Foundation of Gansu Province(No.20JR10RA727)。

摘　　要：Objective:To evaluate the protective effect and mechanism of clemastine fumarate(CLE)on acute lung injury(ALI)in intestinal ischemia-reperfusion(I/R)mice.Methods:Twenty-four SPF Balb/c mice were randomly divided into sham operation group(sham group),ischemia-reperfusion group(I/R group),and clemastine fumarate pretreatment group(I/R+C group).In the I/R group,an intestinal ischemia-reperfusion model was established(ischemia for 40 minutes,reperfusion for 2 hours).In the I/R+C group,CLE 5 mg/kg was intraperitoneally injected before the operation.Lung tissue morphology was observed and scored by HE staining;and the ratios of wet weight to dry weight(W/D)were recorded.the levels of MDA,SOD,GSH-px,NF-κB and TNF-αin lung tissue of each group were determined by ELISA;Western blot method was used to determine the expression of TLR4 protein in lung tissue.Results:Compared with the Sham group,the I/R group had significantly higher lung tissue injury score and wet/dry ratio(P<0.05),increased lung tissue MDA level(P<0.05),decreased SOD and GSH-px levels(P<0.05),and increased NF-κB and TNF-αlevels,the expression of TLR4 protein in lung tissue increased(P<0.05);compared with the I/R group,the lung tissue injury score and wet/dry ratio of the I/R+C group decreased(P<0.05),the level of MDA in lung tissue decreased(P<0.05),the levels of SOD and GSH-px increased(P<0.05),and the levels of NF-κB and TNF-毩decreased(P<0.05),the expression of TLR4 protein in lung tissue decreased(P<0.05).Conclusion:Clemastine fumarate can alleviate acute lung injury after intestinal ischemia-reperfusion in mice,and the mechanism may be related to the inhibition of oxidative stress and inflammatory response in lung tissue.

关 键 词：Clemastine fumarate Reperfusion injury INTESTINAL Acute lung injury 

分 类 号：R574[医药卫生—消化系统]