应用9.4T磁共振CSI技术定量评价小鼠肝脏脂肪含量的价值  

Application of CSI at 9.4T for quantitative assessment of liver fat content in mice

作　　者：沈子剑 马慧 魏述航 隋欣孜 刘楷文 丛岚青 王锡明 王涛 赵新亚 SHEN Zijian;MA Hui;WEI Shuhang;SUI Xinzi;LIU Kaiwen;CONG Lanqing;WANG Ximing;WANG Tao;ZHAO Xinya(Department of Medical Imaging,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;Department of Gastroenterology,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China)

机构地区：[1]山东第一医科大学附属省立医院医学影像科,山东济南250021 [2]山东第一医科大学附属省立医院消化内科,山东济南250021

出　　处：《医学影像学杂志》2022年第11期1966-1970,共5页Journal of Medical Imaging

基　　金：山东省自然科学基金面上项目(编号:ZR2020MH285)。

摘　　要：目的探讨9.4T磁共振化学位移成像(chemical shift imaging,CSI)技术测定小鼠肝脏脂肪含量(LFC)的价值。方法将20只小鼠平均分为实验组与对照组。小鼠先行CSI与磁共振波谱扫描测定LFC,再行病理学检查,以计算脂肪变的肝细胞比例。分析各种脂肪含量测量方法的相关性与一致性。结果CSI与磁共振波谱测量实验组小鼠与对照组小鼠LFC中位数(四分位数间距)分别为35.5%(44.0%)、3.0%(3.3%)和33.5%(44.0%)、2.5%(2.3%);病理学测量实验组小鼠与对照组小鼠脂肪变的肝细胞比例中位数(四分位数间距)分别为54.0%(54.0%)和1.5%(1.3%)。CSI与磁共振波谱测量的肝脏脂肪含量呈正相关(r=0.979,P<0.05),CSI与病理学测量脂肪变的肝细胞比例呈正相关(r=0.974,P<0.05),且有良好的一致性。结论应用9.4T磁共振CSI技术可以准确定量测量小鼠LFC。Objective To investigate the value of chemical shift imaging(CSI)at 9.4T for quantitative assessment of liver fat content in mice.Methods A toal of 20 mice were equally divided into an experimental group and a control group.In both groups,CSI and Magnetic Resonance Spectroscopy scans at 9.4T were performed to determine the liver fat content,and then,histopathological examination was performed to calculate the proportion of hepatocytes with steatosis.The correlations and consistency among the methods were analyzed.Results The median(interquartile interval)of liver fat content with CSI in experimental group and control group was 35.5%(44.0%)and 3.0%(3.3%),respectively.The median(interquartile spacing)of liver fat content with magnetic resonance spectroscopy was 33.5%(44.0%)and 2.5%(2.3%),respectively.The median(interquartile spacing)proportions of hepatocytes with steatosis were 54.0%(54.0%)and 1.5%(1.3%)in the experimental and control group,respectively.The analysis demonstrated a positive correlation between CSI and magnetic resonance spectroscopy etic measurements of liver fat content(r=0.979,P<0.05)and a positive correlation between CSI and the proportion of hepatocytes with steatosis(r=0.974,P<0.05).The analysis showed excellent agreement between the two methods of CSI and histopathology.Conclusion The CSI technique can accurately and quantitatively measure the fat content of mouse liver in 9.4T MR.

关 键 词：脂肪肝 磁共振成像 磁共振波谱 化学位移成像 

分 类 号：R575.5[医药卫生—消化系统] R445.2[医药卫生—内科学]