八角枫及其亚种叶绿体基因组序列结构及系统发育分析  

Sequence structure and phylogenetic analysis of the chloroplast genomes of Alangium chinense(Lour.)Harms and its different subspecies

作　　者：杨小英 刘畅 曾宪法 刘雄伟 赵杰宏 俸婷婷 周英 YANG Xiao-ying;LIU Chang;ZENG Xian-fa;LIU Xiong-wei;ZHAO Jie-hong;FENG Ting-ting;ZHOU Ying(Research Center for Application and Development of Medicinal and Food Resources,College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,药食两用资源应用与开发研究中心,贵州贵阳550025

出　　处：《药学学报》2022年第10期3229-3239,共11页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划项目(2018YFC1708100);贵州省科技计划项目(黔科合平台人才[2019]5407);贵州省“十四五”中医药、民族医药重点学科[QZYYZDXK(JS)-2021-03];贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2021-098);贵州中医药大学2021年度科研创新和探索专项(2018YFC170810101,2018YFC170810201)。

摘　　要：八角枫为八角枫科Alangiaceae八角枫属Alangium常用药用植物,是贵州特色苗药之一,该属植物分化强烈、形态变异复杂,各药材活性成分和药效存在显著差异。本文利用Illumina高通量测序技术对八角枫亚种(稀花八角枫、伏毛八角枫)和毛八角枫叶绿体基因组进行测序,完成其组装注释与结构特征解析,并从NCBI下载同属植物八角枫、高山八角枫进行基因组比较分析。最后,选择NCBI中已发表的与八角枫科亲缘关系较近的物种进行系统发育树构建。结果如下:稀花八角枫、伏毛八角枫和毛八角枫叶绿体基因组全长为156670、156672、156871 bp,为典型的四段式结构,均包含1个长单拷贝区(LSC)、1个短单拷贝区(SSC)及2个反向重复区(IRa和IRb)。均注释到133个基因,其中包括88个蛋白编码基因、37个tRNA基因和8个rRNA基因。叶绿体基因组中均发现了正向重复、回文重复和反向重复3种类型的长重复序列和SSR位点。基因组比较分析表明5个物种边界过渡区域存在多样性,叶绿体基因组未发现重排或倒置,在ndhA、ycf1、rpl16、ycf2及petD等基因的编码区存在显著差异,这些位点为八角枫的分子鉴别提供了新位点。系统分析中毛八角枫单独聚为一支,稀花八角枫和伏毛八角枫聚为一支,支持率为93,亲缘关系较近,表明系统发育树可用于物种鉴别。本文可为八角枫属分类鉴定、保证八角枫药材临床用药安全及规范八角枫药材市场提供依据。Alangium chinense is a commonly used medicinal plant of Alangiaceae Alangium,one of the characteristic Miao medicines in Guizhou.The genus is strongly differentiated and has complex morphological variation,with significant differences in active ingredients and potency among herbs.In this paper,we sequenced the chloroplast genomes of Alangium chinense subsp.Pauciflorum,Alangium chinense subsp.Strigosum and Alangium kurzii Craib using Illumina high-throughput sequencing technology.The genomes of Alangium chinense subsp.Pauciflorum,Alangium chinense subsp.Strigosum and Alangium kurzii Craib chloroplasts were sequenced,their assembly annotation and structural characterization were completed,and the genomes of the congeners Alangium chinense and Alangium alpinum were downloaded from NCBI for comparative analysis.Finally,the phylogenetic tree was constructed by selecting published species with close affinity to Alangium chinense family from NCBI.The results were as follows:Alangium chinense subsp.Pauciflorum,Alangium chinense subsp.Strigosum and Alangium kurzii Craib chloroplast genomes were 156670,156672 and 156871 bp in length,with a typical four-segment structure,all containing one long single copy region(LSC),one short single copy region(SSC)and two inverted repeat regions(IRa and IRb).All of them were annotated to 133 genes,including 88 protein-coding genes,37tRNA genes and 8 rRNA genes.Three types of long repeat sequences and SSR loci,forward repeats,palindrome repeats and reverse repeats,were found in all chloroplast genomes.Comparative genomic analysis showed that there was diversity in the boundary transition regions of the five species,no rearrangements or inversions were found in the chloroplast genome,and there were significant differences in the coding regions of ndhA,ycf1,rpl16,ycf2,and petD genes,which provided new loci for molecular identification of Patagonia.In the phylogenetic analysis,Alangium kurzii var.kurzii clustered as a single species,and Alangium chinense subsp.Pauciflorum and Alangium chinen

关 键 词：八角枫 亚种 叶绿体基因组 重复序列 系统发育 

分 类 号：R931[医药卫生—生药学]