The unconventional prefoldin RPB5 interactor mediates the gravitropic response by modulating cytoskeleton organization and auxin transport in Arabidopsis  

作　　者：Yi Yang Fang Liu Le Liu Mingyue Zhu Jinfeng Yuan Yan‐Xia Mai Jun‐Jie Zou Jie Le Yonghong Wang Klaus Palme Xugang Li Yong Wang Long Wang 

机构地区：[1]State Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Daizong Street 61,Tai'an 271018,China [2]Shandong Provincial Key Laboratory of Biophysics,Institute of Biophysics,Dezhou University,Dezhou 253023,China [3]Sino‐German Joint Research Center on Agricultural Biology,College of Life Sciences,Shandong Agricultural University,Daizong Street 61,Tai'an 271018,China [4]Faculty of Biology,Institute of Biology II/Molecular Plant Physiology,Albert‐Ludwigs‐University of Freiburg,Schänzlestrasse 1Freiburg D‐79104,Germany [5]National Key Laboratory of Plant Molecular Genetics(NKLPMG),CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology(SIPPE),Chinese Academy of Sciences(CAS),Shanghai 200032,China [6]Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China [7]Key Laboratory of Plant Molecular Physiology,CAS Center for Excellence in Molecular Plant Sciences,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [8]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Integrative Plant Biology》2022年第10期1916-1934,共19页植物学报（英文版）

基　　金：supported by an initiation fund from the Shandong Agricultural University,the National Natural Science Foundation of China(31570291);the Shandong“Foreign experts double hundred”Program(WST2017008);the Natural Science Foundation of Shandong Province(ZR2021MC175)。

摘　　要：Gravity-induced root curvature involves the asymmetric distribution of the phytohormone auxin.This response depends on the concerted activities of the auxin transporters such as PIN-FORMED(PIN)proteins for auxin efflux and AUXIN RESISTANT 1(AUX1)for auxin influx.However,how the auxin gradient is established remains elusive.Here we identified a new mutant with a short root,strong auxin distribution in the lateral root cap and an impaired gravitropic response.The causal gene encoded an Arabidopsis homolog of the human unconventional prefoldin RPB5 interactor(URI).At URI interacted with prefoldin 2(PFD2)and PFD6,twoβ-type PFD members that modulate actin and tubulin patterning in roots.The auxin reporter DR5_(rev):GFP showed that asymmetric auxin redistribution after gravistimulation is disordered in aturi-1 root tips.Treatment with the endomembrane protein trafficking inhibitor brefeldin A indicated that recycling of the auxin transporter PIN2 is disrupted in aturi-1 roots as well as in pfd mutants.We propose that At URI cooperates with PFDs to recycle PIN2and modulate auxin distribution.

关 键 词：ARABIDOPSIS auxin CYTOSKELETON PIN2 prefoldin(PFD) unconventional prefoldin RPB5 interactor(URI) 

分 类 号：Q945[生物学—植物学]