地黄生长过程中根际土壤挥发性有机物与细菌群落特征分析  

Analysis of Characteristics of Volatile Organic Compounds and Bacterial Communities in Rhizosphere Soil During Growth of Rehmanniae Radix

作　　者：李伟峰[1] 耿晓桐 龚海燕[1] 王丰青[3] 雷敬卫[1] 谢彩侠[1] 张重义[4] LI Weifeng;GENG Xiaotong;GONG Haiyan;WANG Fengqing;LEI Jingwei;XIE Caixia;ZHANG Zhongyi(Henan Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,China;Xinyang Agriculture and Forestry University,Xinyang 464000,China;Henan Agricultural University,Zhengzhou 450046,China;Fujian Agriculture and Forestry University,Fuzhou 350021,China)

机构地区：[1]河南中医药大学河南省中药质量控制与评价工程技术研究中心,郑州450046 [2]信阳农林学院,河南信阳464000 [3]河南农业大学,郑州450046 [4]福建农林大学,福州350021

出　　处：《中国实验方剂学杂志》2022年第22期149-158,共10页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(82073952,81872950)。

摘　　要：目的:分析地黄生长过程中根际土壤挥发性有机物(VOCs)、细菌群落特征的变化,以及VOCs与细菌群落结构的关系,为地黄连作土壤形成过程中的特征评价及连作土壤微生物的调控研究奠定基础。方法:以地黄7月、8月、10月3个主要生长时期的根际土壤为研究对象,采用气相色谱-质谱法(GC-MS)测定根际土壤乙酸乙酯部位及二氯甲烷部位中VOCs的相对含量,运用高通量测序法测定其根际细菌群落结构特征,利用SPSS 24、SIMCA 14.1等分析3个时期根际土壤VOCs、细菌群落结构的差异性,筛选引起其差异的主要VOCs和标志性菌群,并分析VOCs与细菌群落结构的相关性。结果:地黄3个生长时期根际土壤不同部位中的VOCs存在差异,其中乙酸乙酯部位的VOCs差异物质有间苯二甲酸二辛酯、2-辛基-1-十二烷醇、对甲苯甲酸-2-乙基己酯,二氯甲烷部位的则包括间苯二甲酸二(2-乙基己基)酯、邻苯二甲酸二(2-丙基戊基)酯、对甲苯甲酸-2-乙基己基酯。地黄从苗期到块根膨大末期,其根际土壤中放线菌门、厚壁菌门和绿弯菌门等优势菌门的相对丰度逐渐降低,而且各生长时期的根际土壤均有可以表征自己特有的细菌群落。相关性分析表明,地黄根际土壤VOCs对根际细菌群落结构有影响,其中以酯类、醇类等成分对根际细菌群落结构的影响较大。结论:在地黄的生长过程中,根际土壤的特征表现为酯类和醇类等主要VOCs含量发生变化和主要有益菌群相对丰度的逐渐降低,而且根际土壤VOCs对细菌群落结构的塑造有一定作用。Objective:To analyze the changes of volatile organic compounds(VOCs)and bacterial community characteristics in rhizosphere soil during the growth of Rehmanniae Radix,as well as the relationship between VOCs and bacterial community structure,so as to lay the foundation for the evaluation of the characteristics of continuous cropping soil and the regulation of continuous cropping soil microorganisms.Method:The rhizosphere soil during the three main growth periods of Rehmanniae Radix in July,August and October was used as the research object.Gas chromatography-mass spectrometry(GC-MS)was used to determine the relative contents of VOCs in ethyl acetate and dichloromethane parts in rhizosphere soil.The characteristics of rhizosphere bacterial community structure was determined by high-throughput sequencing,SPSS 24,SIMCA 14.1 and other software were used to analyze the differences of VOCs and bacterial community structure in rhizosphere soil of the three periods,and the main VOCs and iconic bacteria that caused the differences were screened,and the correlation between VOCs and bacterial community structure was analyzed.Result:There were differences in VOCs in different parts of rhizosphere soil during the three growth stages of Rehmanniae Radix.Among them,and the main VOCs that cause differences were dioctyl isophthalate,2-octyl-1-dodecanol and 2-ethylhexyl p-toluic acid in the ethyl acetate part,and 1,3-benzenedicarboxylic acid,di(2-propylpentyl)phthalate and 2-ethylhexyl p-toluenecarboxylic acid in the dichloromethane part.From the seedling stage to the end of tuber enlargement of Rehmanniae Radix,the relative abundance of dominant bacteria such as Actinobacteria,Firmicutes and Chloroflexi in the rhizosphere soil was gradually decreased,and there were unique bacterial communities in rhizosphere soil of each growth stage.Correlation analysis showed that the VOCs in rhizosphere soil of Rehmanniae Radix had an impact on the rhizosphere bacterial community structure,especially the components of esters and alcohols.Conclu

关 键 词：地黄 根际土壤 气相色谱-质谱法(GC-MS) 细菌群落特征 挥发性有机物 高通量测序法 连作障碍 

分 类 号：R22[医药卫生—中医基础理论] R28[医药卫生—中医学] R931[理学—分析化学] O657[理学—化学]