溶剂极性对沙棘渣提取物组成及体外降血糖、降血脂活性的影响  

Effect of solvent polarity on composition, in vitro hypoglycemic and hypolipidemic activities of extracts from seabuckthorn(Hippophae rhanmoides L.) residue

作　　者：王旭 孔志强 赵玉红[1,2] WANG Xu;KONG Zhiqiang;ZHAO Yuhong(School of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China;Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]黑龙江省森林食品资源利用重点实验室,黑龙江哈尔滨150040

出　　处：《精细化工》2022年第10期2060-2068,共9页Fine Chemicals

基　　金：黑龙江省应用技术研究与开发计划项目(GA19C106)。

摘　　要：采用不同极性溶剂经超声波辅助对沙棘果渣和籽渣进行了提取,评价了沙棘渣中活性成分含量及其体外降血糖和降血脂能力,结合超高效液相色谱串联四极杆静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap HRMS)对沙棘渣中活性成分进行了鉴定。结果表明,溶剂极性对提取物得率及降血糖、降血脂能力有显著影响,其中由体积分数为60%乙醇水溶液(记为60%乙醇,下同)提取的提取物具有最高的提取物得率,果渣提取物中总多酚含量[(11.08±0.29) mg/g]、总黄酮含量[(6.49±0.17) mg/g]和原花青素含量[(6.58±0.20) mg/g]最高。籽渣的80%乙醇提取物中总多酚含量[(25.70±0.18) mg/g]最高,籽渣丙酮提取物中总黄酮含量[(18.25±0.19) mg/g]、原花青素含量[(26.98±0.26)mg/g]最高。沙棘果渣和籽渣丙酮提取物具有最好的α-葡萄糖苷酶与α-淀粉酶活性抑制能力。籽渣80%乙醇提取物、果渣60%乙醇提取物具有最好的甘氨胆酸钠和牛磺胆酸钠结合能力。原花青素是籽渣提取物体外降血脂、降血糖活性主要贡献者。果渣提取物中的总多酚与降血脂能力显著相关。从沙棘渣提取物中共鉴定出37种化合物,主要为黄酮和萜类成分。Active components were extracted from seabuckthorn residue by solvents with different polarity via ultrasonic assistance, and identified by ultra-high performance liquid chromatography tandem quadrupole electrostatic field orbital trap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS),followed by evaluation of their content and in vitro hypoglycemic and hypolipidemic activities to explore the influence of solvent polarity. The results showed that solvent polarity had significant effect on extraction yield of extracts, and their hypoglycemic and hypolipidemic performance. Highest yield of extract was obtained using volume fraction 60% aqueous ethanol(denoted as 60% ethanol, the same below),while extract from fruit residue by 60% ethanol had the highest total polyphenol content [(11.08±0.29)mg/g], total flavonoids content [(6.49±0.17) mg/g] and proanthocyanidin content [(6.58± 0.20) mg/g]. The total polyphenol content [(25.70±0.18) mg/g] in extract from seed residue by 80% ethanol was the highest,and the total flavonoids content [(18.25±0.19) mg/g] and proanthocyanidins content [(26.98±0.26) mg/g] in extract by acetone were the highest. Extracts from seabuckthorn fruit and seed residue by acetone displayed best inhibitory activities for α-glucosidase and α-amylase. Extracts from seed residue by 80% ethanol and from fruit residue by 60% ethanol exhibited best binding ability to sodium glycinate and sodium taurocholate. It was also found that procyanidins were the main contributors to the in vitro hypoglycemic and hypolipidemic activities, and the total polyphenols in fruit residue extract were significantly correlated with hypolipidemic capacity. A total of 37 compounds, mainly flavonoids and terpenes, identified.

关 键 词：沙棘渣 活性成分 降血糖作用 降血脂作用 中药现代化技术 

分 类 号：TQ460.1[化学工程—制药化工]