冬虫夏草提取物通过抑制PI3K/Akt通路抑制HBx诱导的系膜细胞增殖和细胞外基质沉积  

Cordyceps sinensis extracts attenuates HBx-induced mesangial cell proliferation and extracellular matrix synthesis by suppression of the PI3K/Akt pathways

作　　者：雷静 何平[1] 蔡文琳 张永哲 张蓓茹[1] 刘大军[1] LEI Jing;HE Ping;CAI Wenlin;ZHANG Yongzhe;ZHANG Beiru;LIU Dajun(Department of Nephrology,Shengjing Hospital of China Medical University,Shenyang 110004,Liaoning,China)

机构地区：[1]中国医科大学附属盛京医院肾内科,辽宁沈阳110004

出　　处：《中国临床药理学与治疗学》2022年第11期1247-1254,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：辽宁省“揭榜挂帅”科技攻关项目(2021JH1/10400050);中国医科大学附属盛京医院345人才项目资助(201940B);辽宁省重点研发计划指导项目(2018225008);辽宁省博士科研启动基金项目(201501005);辽宁省教育厅科研项目(L2013295)。

摘　　要：目的:研究冬虫夏草(cordyceps sinensis)提取物对乙肝病毒X蛋白(hepatitis B virus X protein,HBx)诱导的人系膜细胞(human mesangial cells,HMCs)增殖和细胞外基质(extracellular matrix,ECM)积聚的作用及潜在机制。方法:用重组表达载体pCMV-HBx稳染人系膜细胞建立HBx过度表达模型,用空载体转染对照组。用MTT法和DNA合成法检测细胞增殖。蛋白质印迹法用于检测磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)信号通路相关蛋白和细胞外基质的表达。结果:HBx转染人系膜细胞可诱导系膜细胞增殖、基质积聚。HBx转染的HMCs可增强系膜细胞PI3K/Akt通路的活性,应用冬虫夏草可抑制这种作用。结论:冬虫夏草可通过抑制PI3K/Akt信号通路减弱HBx诱导的人系膜细胞增殖和细胞外基质的产生。AIM:To investigate the effect of C.sinensis extracts on HBx(Hepatitis B virus X protein)induced cell proliferation and extracellular matrix(ECM)accumulation and the underlying mechanism in cultured human mesangial cells(HMCs).METHODS:Human mesangial cells were stable transfected with pCMV-HBx to establish an HBx over-expression model,and a control group was transfected with an empty vector.Cell proliferation was determined by MTT assay and DNA synthesis assay.Western blotting was used to measure the expression of PI3K/Akt signaling pathway-related proteins and extracellular matrix.RESULTS:HBx transfection induced cell proliferation,matrix accumulation.HBx-transfected mesangial cells had increased activity of the PI3K/Akt pathways,and treatment with C.sinensis suppressed this effect.CONCLUSION:C.sinensis attenuates the HBx-induced human mesangial cell proliferation and matrix production in human mesangial cells via inhibition of the PI3K/Akt pathways.

关 键 词：冬虫夏草 增殖 基质沉积 PI3K/AKT HBX 人系膜细胞 

分 类 号：R965.2[医药卫生—药理学]