黄芩素在伊立替康抑制结肠癌细胞增殖中的作用研究  

Baicalein enhances inhibitory effect of irinotecan on proliferation of colon cancer cells

作　　者：孟祥彩 刘洋[1] 武玉 刘振方 王益民[1] MENG Xiangcai;LIU Yang;WU Yu;LIU Zhenfang;WANG Yimin(First Hospital of Qinhuangdao,Qinhuangdao 066000,China)

机构地区：[1]秦皇岛市第一医院,河北秦皇岛066000 [2]石家庄市中医院,河北石家庄050051

出　　处：《陕西中医》2022年第12期1682-1685,共4页Shaanxi Journal of Traditional Chinese Medicine

基　　金：河北省医学科学研究课题(20201428)。

摘　　要：目的:研究黄芩素在伊立替康抑制结肠癌细胞增殖中的作用及相关机制。方法:用结肠癌HT-29细胞作为研究对象。以50、100、200μmol/L黄芩素、5μmol/L伊立替康分别处理结肠癌HT-29细胞。MTT实验和平板克隆实验检测细胞增殖和克隆形成能力;Western blotting法实验检测Src激酶、Yes相关蛋白(YAP)及大肿瘤抑制因子激酶1(LATS1)磷酸化蛋白表达;免疫荧光实验检测细胞YAP表达分布。结果:50μmol/L黄芩素组、100μmol/L黄芩素组、200μmol/L黄芩素组均能增加伊立替康对HT-29细胞的增殖和克隆抑制作用。50μmol/L黄芩素组、100μmol/L黄芩素组、200μmol/L黄芩素组均可下调结肠癌HT-29细胞p-Src蛋白表达,上调p-YAP、p-LATS1蛋白表达。200μmol/L黄芩素组能增加结肠癌HT-29细胞的细胞质YAP分布。结论:黄芩素通过抑制Src-YAP信号通路增强伊立替康对结肠癌细胞的抑制作用。Objective:To study effect of baicalein on irinotecan’s inhibition of colon cancer cell proliferation and related mechanisms.Methods:Colon cancer HT-29 cells were used as research objects.Cells were treated with 50μmol/L baicalein,100μmol/L baicalein,200μmol/L baicalein,5μmol/L irinotecan.MTT assay and plate cloning assay were used to detect cell proliferation and clone formation.Western blotting assay was used to detect expression of Src kinase,Yes-related protein(YAP)and large tumor suppressor kinase 1(LATS1)phosphorylated protein.Immunofluorescence assay was to detect distribution of YAP expression in cells.Results:50μmol/L baicalein,100μmol/L baicalein,200μmol/L baicalein could increase inhibitory effect of irinotecan on proliferation and colony formation of HT-29 cells.50μmol/L baicalein,100μmol/L baicalein,200μmol/L baicalein could down-regulate p-Src protein expression and up-regulate p-YAP,p-LATS1 protein expression in HT-29 cells.200μmol/L baicalein can increase distribution of cytoplasmic YAP in HT-29 cells.Conclusion:Baicalein may enhance the inhibitory effect of irinotecan on colon cancer cell proliferation by inhibiting Src-YAP signaling pathway.

关 键 词：结肠癌 黄芩素 伊立替康 SRC激酶 Yes相关蛋白 细胞增殖 

分 类 号：R735.35[医药卫生—肿瘤]