丹参酮ⅡA磺酸钠的电化学测定及与DNA作用的研究  

Electrochemical determination of sodium tanshinoneⅡA sulfonate and its effect on DNA

作　　者：孙章华 陈美凤 马心英 秦佳濛 薛守庆 SUN Zhang-hua;CHEN Mei-feng;MA Xin-ying;QIN Jia-meng;XUE Shou-qing(Shandong Heze Ecology and Environment Monitoring Center,Heze 274000,China;College of Chemistry and Chemical Engineering,Heze University,Heze 274000,Chin)

机构地区：[1]山东省菏泽生态环境监测中心,山东菏泽274000 [2]菏泽学院化学化工学院,山东菏泽274000

出　　处：《现代化工》2022年第11期256-260,共5页Modern Chemical Industry

基　　金：山东省高等学校科技计划项目(J17KB62)。

摘　　要：制备了聚L-亮氨酸修饰玻碳电极,并对丹参酮ⅡA磺酸钠进行测定。结果表明,在此修饰电极上聚L-亮氨酸催化了丹参酮ⅡA磺酸钠电化学氧化还原反应。实验对该电极的制备条件以及测试条件进行了优化。丹参酮ⅡA磺酸钠的氧化峰电流与浓度呈线性关系,该修饰电极具有良好的灵敏度、选择性和稳定性,可用于相关产品的生产及质量控制。另外,用电化学和紫外分光光度法分别研究了丹参酮ⅡA磺酸钠和DNA的相互作用。结果表明,丹参酮ⅡA磺酸钠和dsDNA发生了明显的相互作用。Poly(L-leucine)modified glassy carbon electrode is prepared,and used to determine sodium tanshinoneⅡA sulfonate.The results show that poly(L-leucine)catalyzes the electrochemical redox reactions of sodium tanshinoneⅡA sulfonate on the modified electrode.The preparation and testing conditions for the electrode are optimized.The oxidation peak current of sodium tanshinoneⅡA sulfonate shows linear with its concentration.This modified electrode has good sensitivity,selectivity and stability,and can be used for the production and quality control of related products.In addition,the interaction between sodium tanshinoneⅡA sulfonate and DNA is studied by means of electrochemistry and UV spectrophotometry,respectively.It is shown that there is obvious interaction between sodium tanshinoneⅡA sulfonate and dsDNA.

关 键 词：L-亮氨酸 玻碳电极 丹参酮ⅡA磺酸钠 

分 类 号：X132[环境科学与工程—环境科学] O657.1[理学—分析化学]