次声对大鼠神经元细胞凋亡与Tau蛋白表达的影响  

Effects of infrasound on neuronal apoptosis and Tau protein expression in rats

作　　者：蔡丽 王晋 张鸽 巩丽 张文东 刘朝晖 CAI Li;WANG Jin;ZHANG Ge(Department of Rehabilitation Medicine,Tangdu Hospital,the Air Force Military Medical University,Xi'an,Shanxi,710038)

机构地区：[1]空军军医大学第二附属医院唐都医院康复医学科,陕西省西安市710038 [2]空军军医大学放射医学教研室 [3]空军军医大学第二附属医院唐都医院病理科

出　　处：《中国康复医学杂志》2022年第11期1453-1456,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金项目(31470825)。

摘　　要：目的:探索声压级水平是8Hz、90dB的次声连续7d和14d暴露对大鼠海马区神经元细胞凋亡与Tau蛋白表达的影响。方法:建立次声暴露模型。将24只SD大鼠平均分为对照组、1d组、7d组和14d组等四组。1d组次声暴露2h,7d组次声暴露7d每天2h,14d组次声暴露14d每天2h。对照组放置于无次声暴露仓内2h。运用TUNEL染色、Western Blot、ELISA等实验方法探索次声对大鼠海马区神经元细胞凋亡与Tau蛋白的影响。结果:连续7d和14d的次声暴露组大鼠海马区神经元细胞凋亡增多,与对照组和1d组相比差异均有显著性意义且Tau蛋白表达也增多,与对照组和1d组相比差异也具有显著性意义,呈现随暴露时间的延长而增多的趋势。结论:连续7d和14d次声暴露可能会导致大鼠神经功能损害。Objective:To study the effects of 7 day and 14 day infrasound exposure on neuronal apoptosis and Tau protein’s expression in rats.Method:Twenty-four male SD rats were randomly divided into control group,one-day infrasound exposure group,seven-day infrasound exposure group and fourteen-day infrasound exposure group,there were six rats in each group.The test groups were put in 8 Hz,90dB infrasound environment two hours per day,and the control group rats were put in the instrument without 8Hz,90dB infrasound exposure two hours.Neuronal apoptosis was detected with TUNEL staining,the expression of tau protein in hippocampus was detected with Western blotting and Elisa.Result:The number of neuronal apoptosis was more than the control group and the 1-day group in 7-day group and 14-day group.The differences were statistically significant.Compared with the control group and the 1-day group,the expression of Tau protein was also increased significantly,which showed a trend of increasing with the extension of exposure time.Conclusion:Seven day and fourteen day 8Hz,90dB infrasound exposure may cause nerve function damage in rats.

关 键 词：次声暴露 凋亡 TAU蛋白 大鼠 海马区 

分 类 号：R741[医药卫生—神经病学与精神病学] R493[医药卫生—临床医学]