低鱼粉饲料中添加羟基蛋氨酸硒对凡纳滨对虾生长性能、抗氧化能力和抗亚硝酸盐胁迫的影响  

Effects of hydroxyl methionine selenium supplementation in low-fishmeal diet on growth,antioxidant ability and nitrite tolerance of Pacific white shrimp(Litopenaeus vannamei)

作　　者：李静 李晓丽 王玲[1] 鲁康乐[1] 宋凯[1] 张春晓[1] LI Jing;LI Xiaoli;WANG Ling;LU Kangle;SONG Kai;ZHANG Chunxiao(Fisheries College of Jimei University,Xiamen Key Laboratory for Feed Quality Testing and Safety Evaluation,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,厦门市饲料质量检测与安全评价重点实验室,福建厦门361021

出　　处：《水产学报》2022年第10期1848-1859,共12页Journal of Fisheries of China

基　　金：福建省水产种子创新与产业化项目(2017FJSCZY02);农业公益科研专项基金(201303053)。

摘　　要：为研究低鱼粉饲料中添加羟基蛋氨酸硒(HMSe)对凡纳滨对虾生长性能、抗氧化能力及抗亚硝酸盐胁迫的影响,实验选用初始体质量为(0.90±0.05)g的凡纳滨对虾,分别投喂HMSe添加水平为0.000、0.375、0.750、1.500和2.250 mg/kg体质量的实验饲料,命名为HMSe0、HMSe1、HMSe2、HMSe3和HMSe4,每组3个重复,养殖8周,养殖实验结束后,进行12 h的亚硝酸盐胁迫实验。结果显示,凡纳滨对虾的终末体质量(FBW)、增重率(WGR)和饲料效率(FE)随着饲料中HMSe水平的增加呈现先升高后下降的趋势,并在HMSe2组达到峰值。随着饲料中HMSe水平的提高,对虾全体和肌肉中的硒含量均显著增加,而全虾粗蛋白和粗脂肪的含量呈现先升高后降低的趋势,并分别在HMSe2组和HMSe1组达到最大值。HMSe4组中凡纳滨对虾的血清谷胱甘肽过氧化物酶(GSH-Px)和肝胰腺过氧化氢酶(CAT)活性显著高于HMSe0组。亚硝酸盐胁迫显著影响凡纳滨对虾的存活率。与胁迫前比较,胁迫后对虾肝胰腺中MDA含量升高,GST、CAT和T-SOD的活性降低,而补充适量的HMSe可减轻这些负面影响。根据凡纳滨对虾胁迫后成活率的二次多项式回归分析,低鱼粉饲料中凡纳滨对虾的HMSe最适添加量为1.350 mg/kg。研究表明,在低鱼粉饲料中添加0.750~1.350 mg/kg HMSe,能够提高凡纳滨对虾的生长性能、饲料利用率和抗亚硝酸盐胁迫的能力。Selenium is one of the important trace elements in aquatic animals,which plays an important role in animal growth and antioxidant ability.Compared with inorganic Se,organic Se is better absorbed and has higher bioavailability and tissue accumulation in many aquatic animals.The present study was performed to investigate the effects of hydroxyl methionine selenium(HMSe)supplementation in low-fishmeal diet on growth performance,antioxidant ability and nitrite tolerance of Litopenaeus vannamei.In this experiment,L.vannamei with an initial body weight of 0.90±0.05 g was fed with low-fishmeal diets(15%fishmeal)supplemented with 0.000,0.375,0.750,1.500 and 2.250 mg/kg HMSe respectively,named HMSe0,HMSe1,HMSe2,HMSe3 and HMSe4.Each group had 3 replicates with 50 shrimp in each tank(500 L).The shrimp was fed to visual satiation three times daily at 08:00,14:00 and 19:00 h for 8 weeks.After the feeding trial,12 h nitrite stress test was conducted.The results showed as follows:The finial body weight(FBW,15.03-16.11 g),weight gain(WG,1570.1-1689.8%)and feed efficiency(FE,1.10-1.33)of L.vannamei increased firstly and then decreased with the increase of dietary HMSe level in the diet,and reached the maximum value in the HMSe2 group.With the increase of the dietary HMSe level,the feeding intake(FI)decreased firstly and then increased.There was no significant difference in protein efficiency ratio(PER)and survival rate(SR)in all groups.With the increase of dietary HMSe level,the contents of selenium in whole body and muscle both increased significantly,while the contents of crude protein and crude fat in L.vannamei increased firstly and then decreased,and reached the maximum in the HMSe2 group and HMSe1 group,respectively.The contents of moisture and ash showed no significant effects in all groups.The activities of serum glutathione peroxidase(GSH-Px)and hepatopancreas catalase(CAT)in the HMSe4 group were significantly higher than those in the HMSe0 group.With the increase of the dietary HMSe level,the activities of total supero

关 键 词：凡纳滨对虾 羟基蛋氨酸硒 成活率 抗氧化能力 亚硝酸盐 胁迫 

分 类 号：S963.7[农业科学—水产养殖]