过表达整合素β6对人结肠癌SW480细胞基因表达的影响  

Overexpression of integrinβ6 on gene expression of human colon cancer SW480 cells

作　　者：薛栋 孙祺 XUE Dong;SUN Qi(Department of General Surgery,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China)

机构地区：[1]西安交通大学第一附属医院普通外科,陕西西安710061

出　　处：《中国临床研究》2022年第11期1503-1509,共7页Chinese Journal of Clinical Research

基　　金：国家自然科学基金(81702362,82073271)。

摘　　要：目的探讨过表达整合素β6对人结肠癌SW480细胞基因表达的影响,揭示整合素β6促进人结肠癌细胞侵袭、转移的潜在机制。方法通过慢病毒载体构建稳定过表达整合素β6的SW480细胞株(SW480/β6-OE)和空载体转染的SW480细胞株(SW480/Mock),western blot检测整合素β6过表达效率,Transwell实验检测过表达整合素β6对SW480细胞侵袭能力的影响。高通量转录组测序技术分析过表达整合素β6后SW480细胞基因表达谱的变化,并对差异表达基因(DEGs)进行GO和KEGG功能富集分析。结果过表达整合素β6显著增强SW480细胞的侵袭能力(P<0.05)。相对于SW480/Mock细胞,在SW480/β6-OE细胞中共筛选出1472个DEGs,其中上调基因840个,下调基因632个。KEGG富集分析显示,DEGs显著富集于癌症通路、MAPK信号通路、PI3K-AKT信号通路、细胞因子与其受体互作、cAMP信号通路和Wnt信号通路等。结论过表达整合素β6促进结肠癌细胞的侵袭、转移,改变结肠癌细胞的基因表达谱,DEGs显著富集的信号通路包括多条已被证实在结肠癌的发生发展中发挥重要作用的信号通路。Objective To explore the effects of overexpression of integrinβ6 on gene expression of human colon cancer SW480 cells to reveal a potential mechanism of integrinβ6 in promoting invasion and metastasis of human colon cancer cells.Methods SW480 cell line stably overexpressing integrinβ6(SW480/β6-OE)and SW480 cells transfected with empty vector(SW480/Mock)were established by lentivirus vector.The overexpression efficacy of integrinβ6 was detected by western blot,and the effect of overexpression integrinβ6 on the invasion ability of SW480 cells was detected by transwell assay.High throughput transcriptome sequencing technology was used to analyze the changes in gene expression profile of SW480 cells after overexpression of integrinβ6,and the GO and KEGG function enrichment analysis was conducted for differentially expressed genes(DEGs).Results Overexpression of integrinβ6 significantly enhanced the invasion ability of SW480 cells(P<0.05).Compared with SW480/Mock cells,1472 DEGs were screened from SW480/β6-OE cells,including 840 up-regulated genes and 632 down-regulated genes.KEGG enrichment analysis revealed that DEGs were enriched in the pathways involved in cancer,mitogen-activated protein kinase(MAPK),PI3K-AKT,cytokine-cytokine receptor interaction,cAMP signal pathway and Wnt signal pathway.Conclusions Overexpression of integrinβ6 promotes the invasion and metastasis of SW480 cells and changes the gene expression profile of colon cancer cells.The multiple signaling pathways in which DEGs are significantly enriched have been confirmed to play an important role in the occurrence and development of colon cancer.

关 键 词：结肠癌 整合素β6 转录组测序 侵袭 差异表达基因 信号通路 

分 类 号：R735.3[医药卫生—肿瘤]