活性炭与白砂糖互作对褐苞薯蓣试管薯诱导的影响  

Interactive Effects of Activated Carbon and White Granule Sugar on the Microtuber Induction of Dioscorea persimilis

作　　者：李丽红 华树妹 陈芝华 李清 邓才生 黄哲鸿 LI Li-hong;HUA Shu-mei;CHEN Zhi-hua;LI Qing;DENG Cai-sheng;HUANG Zhe-hong(Sanming Agricultural Science Institute,Shaxian 365051,PRC)

机构地区：[1]三明市农业科学研究院,福建沙县365051

出　　处：《湖南农业科学》2022年第11期15-18,共4页Hunan Agricultural Sciences

基　　金：福建省引导性项目(2019N0045);三明市科技计划项目(2021-N-9)。

摘　　要：以清流雪薯、明溪淮山组培试管苗为材料,用白砂糖取代蔗糖,研究不同浓度的活性炭和白砂糖互作对其试管薯诱导的影响。结果表明:在一定范围内,随白砂糖添加量的增加,试管薯单薯重呈明显上升趋势;不同品种对活性炭与白砂糖互作的反应不同,清流雪薯试管薯诱导的最适培养基为MS+KT 2.0 mg/L+NAA 0.2 mg/L+活性炭1.0 g/L+白砂糖40 g/L,诱导率最高,达87.5%;平均单薯重为0.49 g;而明溪淮山试管薯诱导的最适培养基为MS+KT 2.0 mg/L+NAA 0.2 mg/L+活性炭0.5 g/L+白砂糖60 g/L,诱导率为70.83%,平均单薯重为0.35 g。Using the in vitro plantlets of Dioscorea persimilis cultivars"Qingliuxueshu"and"Mingxihuaishan"as experimental materials,this paper aimed to study the effects of interaction between different concentrations of white granule sugar(instead of sucrose)and activated carbon on the microtuber induction.The results showed that the weight of single tuber and the proportion of effective tubers significantly increased with the increase of white granule sugar concentration within a certain range.Different varieties had different reactions to the interaction between white granule sugar and activated carbon.MS basal medium supplemented with KT 2.0 mg/L,NAA 0.2 mg/L,activated carbon 1.0 g/L and white granule sugar 40 g/L was the optimum for the microtuber induction of"Qingliuxueshu",with a highest induction rate of 87.5%and 0.49 g average single tuber weight;the optimum medium for the microtuber induction of"Mingxihuaishan"contained MS basal medium,KT 2.0 mg/L,NAA 0.2 mg/L,activated carbon 0.5 g/L and white granule sugar 60 g/L,achieving a 70.83%induction rate and 0.35 g average single tuber weight.

关 键 词：活性炭 白砂糖 褐苞薯蓣 试管薯诱导 

分 类 号：S632.1[农业科学—蔬菜学]