Circular RNA circCCNB1 inhibits the migration and invasion of nasopharyngeal carcinoma through binding and stabilizing TJP1 mRNA  

作　　者：Mengyao Zhao Yian Wang Fenghua Tan Lingyun Liu Xiangchan Hou Chunmei Fan Le Tang Yongzhen Mo Yumin Wang Qijia Yan Zhaojian Gong Zheng Li Qianjin Liao Can Guo He Huang Xi Zeng Guiyuan Li Zhaoyang Zeng Wei Xiong Fuyan Wang 

机构地区：[1]NHC Key Laboratory of Carcinogenesis,Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha 410078,China [2]Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education,Cancer Research Institute,Central South University,Changsha 410083,China [3]Cancer Research Institute,Hengyang Medical College,University of South China,Hengyang 421009,China [4]Department of Oral and Maxillofacial Surgery,the Second Xiangya Hospital,Central South University,Changsha 410011,China [5]Hunan Key Laboratory of Nonresolving Inflammation and Cancer,Disease Genome Research Center,the Third Xiangya Hospital,Central South University,Changsha 410013,China

出　　处：《Science China(Life Sciences)》2022年第11期2233-2247,共15页中国科学（生命科学英文版）

基　　金：the National Natural Science Foundation of China(82002239,82072374 and 82073135);the Overseas Expertise Introduction Project for Discipline Innovation(111 Project,111-2-12);the Natural Science Foundation of Hunan Province(2021JJ41043 and 2021JJ30897);Central South University Graduate Research and Innovation Project(2021zzts0310)。

摘　　要：Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(circ RNAs)exacerbate the process of metastasis in NPC;however,their underlying mechanisms remain unclear.We found that the circular RNA circ CCNB1,encoded by the oncogene CCNB1,was downregulated in NPC biopsies and cell lines.In vitro assays show that circ CCNB1 inhibits NPC cell migration and invasion.Moreover,circ CCNB1 induces a protein,nuclear factor 90(NF90),to bind and prolong the half-life of tight junction protein 1(TJP1)m RNA.Upregulation of TJP1 enhances tight junctions between cancer cells and inhibits NPC cell migration and invasion.This study reveals a novel biological function of circ CCNB1 in the migration and invasion of NPC by enhancing the tight junctions of cancer cells by binding to NF90 proteins and TJP1 m RNA,and may provide a potential therapeutic target for NPC.

关 键 词：nasopharyngeal carcinoma circCCNB1 NF90 TJP1 migration and invasion 

分 类 号：R739.63[医药卫生—肿瘤]