微小RNA-214-5p靶向SUZ12对宫颈肿瘤细胞增殖和侵袭能力的影响  

Effect of microRNA-214-5p targeting SUZ12 on proliferation and invasion of cervical cancer cells

作　　者：杨婷婷[1] 林春丽[2] 王爱华[1] 朱丽娟[1] Yang Tingting;Lin Chunli;Wang Aihua;Zhu Lijuan(Shangqiu First People′s Hospital,Department of Gynecologic Oncology,Shangqiu Clinical College,Xuzhou Medical University,Shangqiu 476000,China;Shangqiu First People′s Hospital,Department of Oncology,Shangqiu Clinical College,Xuzhou Medical University,Shangqiu 476000,China)

机构地区：[1]商丘市第一人民医院徐州医科大学商丘临床学院妇瘤科,476000 [2]商丘市第一人民医院徐州医科大学商丘临床学院肿瘤科,476000

出　　处：《中华实验外科杂志》2022年第10期1968-1971,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨微小RNA(miR)-214-5p靶向SUZ12对宫颈癌细胞增殖和侵袭能力的影响。方法选取2020年6月到2022年6月商丘市第一人民医院收治的宫颈癌组织和癌旁组织作为研究对象,采用荧光定量聚合酶链反应(PCR)分析miR-214-5p表达水平。人宫颈癌细胞株CaSki随机分为对照组和miR-214-5p组。分别采用对照慢病毒和miR-214-5p慢病毒感染建立稳定细胞株。采用细胞计数试剂盒(CCK-8)和BrdU实验分析两组细胞的增殖活性;采用划痕实验分析细胞的迁移能力;采用Transwell分析两组细胞的侵袭能力。通过数据库和双荧光素酶报告基因分析miR-214-5p的靶基因。采用蛋白质免疫印迹(Western blot)分析靶蛋白的表达水平。组间计量数据比较采用t检验。结果癌旁组织中miR-214-5p表达水平(1.37±0.13)明显高于宫颈癌组织(0.56±0.14),差异有统计学意义(t=32.110,P<0.05)。对照组细胞miR-214-5p表达水平(1.16±0.08)明显低于miR-214-5p组细胞(2.79±0.18),差异有统计学意义(t=19.300,P<0.05)。对照组细胞吸光度(A)值(2.79±0.18)明显高于miR-214-5p组细胞(1.16±0.08),差异有统计学意义(t=19.300,P<0.05)。对照组细胞BrdU阳性率[(55.83±4.00)%]明显高于miR-214-5p组细胞[(28.29±3.90)%],差异有统计学意义(t=12.080,P<0.05)。对照组细胞划痕愈合率[(80.98±5.36)%]明显高于miR-214-5p组细胞[(47.81±3.11)%],差异有统计学意义(t=13.120,P<0.05)。对照组细胞侵袭细胞数量[(124.17±12.22)个]明显高于miR-214-5p组细胞[(72.83±10.78)个],差异有统计学意义(t=7.716,P<0.05)。SUZ12是miR-214-5p的靶基因。对照组细胞SUZ12蛋白表达水平(1.10±0.08)明显高于miR-214-5p组细胞(0.46±0.08),差异有统计学意义(t=13.730,P<0.05)。结论miR-214-5p在宫颈癌组织中呈低表达,通过靶向调控SUZ12蛋白,调控着宫颈癌细胞的增殖、迁移和侵袭过程。Objective To investigate the effect of microRNA(miR)-214-5p targeting SUZ12 on the proliferation and invasion of cervical cancer cells.Methods The expression level of miR-214-5p was analyzed by fluorescence quantitative polymerase chain reaction(PCR)in cervical cancer tissues and adjacent tissues treated in our hospital from June 2020 to June 2022.Human cervical cancer cell line CaSki was randomly divided into control group and miR-214-5p group.Stable cell lines were established by infection with control lentivirus and miR-214-5p lentivirus,respectively.The proliferative activity of the two groups of cells were analyzed by cell counting kit-8(CCK-8)and BrdU assay.The migration ability of cells was analyzed by scratch test.The invasiveness of the two groups of cells was analyzed by Transwell.The target genes of miR-214-5p were analyzed by database and dual luciferase reporter gene.The expression levels of target proteins were analyzed by Western blotting.T test was used to compare the measurement data between groups.Results The expression level of miR-214-5p in adtissues(1.37±0.13)was significantly higher than that in cervical cancer tissues(0.56±0.14,t=32.110,P<0.05).The expression level of miR-214-5p in control group(1.16±0.08)was significantly lower than that in miR-214-5p group(2.79±0.18,t=19.300,P<0.05)The absorbance(A)value of the cells in the control group(2.79±0.18)was significantly higher than that of the miR-214-5p group(1.16±0.08,t=19.300,P<0.05)The BrdU positive rate of cells in the control group[(55.83±4.00)%]was significantly higher than that of cells in the miR-214-5p group[(28.29±3.90)%,t=12.080,P<0.05].The wound healing rate of cells in the control group[(80.98±5.36)%]was significantly higher than that of cells in the miR-214-5p group[(47.81±3.11)%,t=13.120,P<0.05].The number of invasive cells in the control group[(124.17±12.22)cells]was significantly higher than that of miR-214-5p group[(72.83±10.78)cells,t=7.716,P<0.05].Suz12 is the target gene of miR-214-5p.The expression level of S

关 键 词：宫颈癌 微小RNA-214-5p SUZ12 增殖 迁移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]