环状RNA circ100367在甲状腺癌中的诊断价值及其与免疫相关因子的关系  

Circular RNA circ_100367 as a diagnostic marker for thyroid cancer and its relationship with immune-related factors

作　　者：师礼鹏[1] 闫慧明[1] 高杰清[2] 商中华[1] 赵舸[1] Shi Lipeng;Yan Huiming;Gao Jieqing;Shang Zhonghua;Zhao Ge(Department of General Surgery,the Second Hospital of Shanxi Medical University,Taiyuan 030001;Department of Endocrinology,Beijing Rehabilitation Hospital,Capital Medical University,Beijing 110004)

机构地区：[1]山西医科大学第二医院普外科,太原030001 [2]首都医科大学附属北京康复医院内分泌科,北京110004

出　　处：《中华内分泌外科杂志》2022年第5期573-578,共6页Chinese Journal of Endocrine Surgery

基　　金：山西省面上青年基金项目(201901D211496)。

摘　　要：目的探讨环状RNA circ00367在甲状腺癌(thyroid carcinoma,THCA)中的诊断价值及其与免疫相关因子的关系。方法根据美国国家生物技术信息中心(National Center for Biotechnology Information,NCBI)网站提供的数据芯片分析THCA中差异表达的circRNAs,将circ00367纳入本次研究。收集175例甲状腺癌患者血清与健康人血清,使用qRT-PCR检测血清样本中circ00367及其线性转录本DCAF8 mRNA的表达水平,计算circ00367与DCAF8的相关性。分析circ00367的表达与患者临床病理特征、免疫浸润水平及免疫抑制因子PD-1的相关性。结果与健康人血清相比(1.00±0.37),甲状腺癌患者血清中circ00367的表达水平(1.37±0.41)显著升高(t=8.80,P<0.001),DCAF8 mRNA表达差异无统计学意义(t=1.67,P=0.095),但circ00367与DCAF8 mRNA表达呈现正相关的关系(r=0.17,P=0.028)。分析circ00367表达与临床病理特征发现,较M0组患者(1.26±0.40),circ00367在M1和Mx中(1.43±0.40)过表达(t=2.63,P=0.009);较N0期患者(1.24±0.36),circ00367在N1和Nx期患者血清(1.45±0.42)中过表达(t=3.48,P=0.001);较淋巴结检测阴性患者的血清(1.28±0.36),circ00367在阳性患者血清中过表达(1.42±0.43)(t=2.14,P=0.034);较T1+T2分期患者(1.30±0.37),T3+T4患者血清中circ00367过表达(1.40±0.43)(t=2.22,P=0.028)。分析circ00367表达与免疫浸润水平发现,高表达的circ00367与CD8+T细胞(r=0.25,P=0.024)、巨噬细胞(r=0.22,P=0.038)、CD4+T细胞(r=0.25,P=0.020)和B细胞(r=0.23,P=0.033)水平具有显著正相关性。circ00367表达与免疫抑制因子PD-1也呈正相关(r=0.19,P=0.011)。结论circ00367可作为甲状腺癌诊断的标志物且其表达与免疫相关因子具有较强关联。Objective To investigate the diagnostic value of circular RNA circ_100367 in thyroid cancer(THCA)and its relationship with immune-related factors.Methods According to the data chip provided by the National Center for Biotechnology Information(NCBI)website,the differentially expressed circRNAs in THCA were analyzed,then circ_100367 was included in this study.The serum of 175 THCA patients and healthy people were collected,and the expression levels of circ_100367 and its linear transcript DCAF8 mRNA in serum samples were detected by qRT-PCR,and the correlation between circ_100367 and DCAF8 was calculated.The correlation between the expression of circ_100367 and the clinicopathological characteristicsof the patients,immune infiltration level and immunosuppressive factor PD-1 was analyzed.Results Compared with serum of healthy people(1.00±0.37),expression level of circ_100367 in serum of THCA patients was significantly increased(1.37±0.41)(t=8.80,P<0.001),and there was no significant difference in DCAF8 mRNA expression(t=1.67,P=0.095),but circ_100367 was positively correlated with DCAF8 mRNA expression(r=0.17,P=0.028).Analysis of expression and clinicopathological characteristics of circ_100367 showed that compared with patients in M0 group(1.26±0.40),circ_100367 was overexpressed in M1 and Mx patients(1.43±0.40)(t=2.63,P=0.009);compared with N0 patients(1.24±0.36),circ_100367 was overexpressed in serum of N1 and Nx patients(1.45±0.42)(t=3.48,P=0.001);compared with serum of patients with negative lymph node detection(1.28±0.36),circ_100367 was overexpressed in serum of positive patients(1.42±0.43)(t=2.14,P=0.034);compared with T1+T2 stage patients(1.30±0.37),circ_100367 expression was overexpressed in serum ofT3+T4 patients(1.40±0.43)(t=2.22,P=0.028).Analysis of the expression and immune infiltration levels of circ_100367 found that highly expressed circ_100367 was associated with CD8+T cells(r=0.25,P=0.024),macrophages(r=0.22,P=0.038),CD4+T cells(r=0.25,P=0.020)and B cell(r=0.23,P=0.033)levels.The expressi

关 键 词：circ100367 DCAF8 免疫浸润 PD-1 甲状腺癌 

分 类 号：R736.1[医药卫生—肿瘤]